Johns Hopkins University

Xiaohui S Lin

William G Nelson

Srinivasan Yegnasubramanian

Zachery Reichert

Traci J Speed

Angelo M DeMarzo

Jila Bakker

Srinivasan Nasubramanian

Julia C Tchou

The present invention provides methods for discovering agents that are effective in reversing epigenetic silencing by inhibiting the interaction of methyl-binding (MBD) proteins with methylated genomic DNA. Also provided are methods for reactivating silenced genes having CpG island hypermethylation along with methods for treatment and prevention of diseases, such as cancer and sickle cell anemia, by administering an agent that modulates methyl-binding domain (MBD) protein-mediated transcriptional repression, thereby increasing gene transcription to prevent or treat disease. Additionally, compounds identified by the present invention useful for treatment and prevention of diseases, such as cancer and sickle cell anemia, are provided.

Agents for reversing epigenetic silencing of genes

The present invention provides methods and kits useful for enriching, identifying and quantifying methylated DNA3 particularly hypermethylated CpG islands by digesting a sample with a methylation-sensitive restriction endonuclease and capturing methylated restriction fragments with a methyl-binding capture reagent. The methods of the invention may be used in the detection of cancer, particularly detection of prostate cancer.

Compare-MS: method rapid, sensitive and accurate detection of DNA methylation

Provided are methods and compositions useful for the diagnosis, prognosis and treatment of hepatic cellular proliferative disorders. The methods include the modulation or analysis of hypemethylated glutathione-S-transferase nuleic acid sequence in hepatic samples and biological fluids.

Methods of diagnosing and treating hepatic cell proliferative disorders

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Redland, CA, United States of America

Xiaohui S Lin