Illumina Cambridge Limited

Illumina Corporation

Gendaq Limited

Solexa, Inc.

Xiaohai Liu

Xiaolin Wu

John Milton

Colin Lloyd Barnes

Silke Ruediger

Antoine Francais

Nikolai Nikolaevich Romanov

Shankar Balasubramanian

Geoffrey Paul Smith

Elena Cressina

Carole Anastasi

Mark Smith

Joseph Brennan

Michael Callingham

Randall Smith

Patrick McCauley

Adam Culley

Angelica Mariani

Helmy A Eltoukhy

Jonathan Mark Boutell

Roberto Rigatti

Robert C Kain

Wenyi Feng

Min-Jui Richard Shen

Harold Philip Swerdlow

Bernard Hirschbein

Tobias William Barr Ost

Anthony Romieu

Kay Klausing

Gerardo Turcatti

David James Earnshaw

Thomas J Joseph

Graham John Worsley

Carolyn Tregidgo

Isabelle Rasolonjatovo

Wayne N George

Niall Hynes

Anno Koetje

Natasha Crake

Andrew Antony Brown

Yen Choo

Maria Candelaria Rogert Bacigalupo

John M Beierle

Brian Douglas Mather

Mark Isalan

Isabelle Marie Julia Rasolonjatovo

Peyton Shieh

Sachin Dinesh Patel

Wouter Meuleman

Rosamond Jackson

Shhankar Balasubramanian

Justyna Piekos

Jie Mao

Pietro Gatti Lafranconi

Elliot John Lawrence

Isabelle Marie J Rasolonjatovo

Thomas Joseph

An example of a kit includes a flow cell and a cleavage mix. An example flow cell includes a substrate; a catalytic polymeric hydrogel on the substrate, the catalytic polymeric hydrogel including a deblocking catalyst; and an amplification primer attached to the catalytic polymeric hydrogel. The deblocking catalyst accelerates cleavage of a blocking group of a 3' OH blocked nucleotide introduced to the flow cell and incorporated into a template strand attached to the amplification primer. An example of the cleavage mix includes a component to initiate cleavage of the blocking group.

Kits and flow cells

The present application relates to substituted coumarin derivatives and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

Substituted coumarin dyes and uses as fluorescent labels

Embodiments of the present disclosure relate to nucleotide with acetal 3'-OH blocking groups. Also provided herein are methods of using fully functionalized nucleotides containing the 3′ acetal blocking group for sequencing applications.

Methods of sequencing using nucleotides with 3' acetal blocking group

Embodiments of the present disclosure relate to nucleotide molecules with a 3' AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3′-OH blocking group for sequencing applications.

Nucleotides with a 3' AOM blocking group

Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.

Cyclooctatetraene containing dyes and compositions

Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by NaSO. Nucleotides and oligonucleotides comprising a 3' phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

Compositions and methods for chemical cleavage and deprotection of surface-bound oligonucleotides

The present disclosure relates to new nucleotide and oligonucleotide compounds and their use in nucleic acid sequencing applications.

Short pendant arm linkers for nucleotides in sequencing applications

The present application relates to substituted dyes containing bis-boron fused heterocycles and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

Fluorescent dyes containing bis-boron fused heterocycles and uses in sequencing

The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

Polymethine compounds and their use as fluorescent labels

The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

Methods and compositions for nucleic acid sequencing

Embodiments of the present disclosure relate to methods of preparing growing polynucleotides using nucleotide molecules with a 3' AOM blocking group. Also provided herein are kits related to such methods.

Methods of preparing growing polynucleotides using nucleotides with 3' AOM blocking group

Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with acetal 3'-OH blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and the uses of fully functionalized nucleotides containing the 3&#x2032; acetal blocking group for sequencing applications.

Nucleosides and nucleotides with 3' acetal blocking group

Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith.

Modified nucleotide linkers

Growing community of inventors

Nr Saffron Walden, United Kingdom

Xiaohai Liu