New England Biolabs, Inc.

Xiaofeng Shi

Christopher H Taron

James C Samuelson

Minyong Chen

Saulius Vainauskas

Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomoleules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.

Universal N-glycan binding reagent

Provided herein is an &#x3b1;-fucosidase that can cleave a conjugate comprising an N-glycan and a label where the label is added by amine reactive chemistry. The &#x3b1;-fucosidase also has an accelerated reaction time using Schiff base labeled N-glycans compared with bovine kidney fucosidase. A reaction mix, enzyme mix and kit comprising the &#x3b1;-fucosidase are provided, as well as a method for analyzing glycoproteins. The &#x3b1;-fucosidase finds particular use in analyzing the N-glycans of therapeutic glycoproteins.

Cleavage of fucose in N-glycans

Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomolecules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.

Growing community of inventors

Beverly, MA, United States of America

Xiaofeng Shi