Mgi Tech Co., Ltd.

Bgi Shenzhen & Bgi Shenzhen Co., Limited

Foshan Electrical and Lighting Co., Ltd

Xiaofang Cheng

Wenwei Zhang

Hui Fu Jiang

Yiguang Zhu

Bin Wei

Ou Wang

Weimao Wang

Luman Cui

Liangying Zou

Cankun Chang

Fei Fan

Wenchao Ding

A method for obtaining a single-cell mRNA sequence, including: (1) capturing mRNA of a cell by using a cell tag carrier, and performing reverse transcription to obtain cDNA having a cell tag; (2) obtaining multiple cDNA fragments having molecular tags by using a transposase complex and a molecular tag carrier; (3) performing high-throughput sequencing; (4) performing sequence assembly according to the molecular tags to obtain the sequence of each mRNA; and () obtaining the sequence of all mRNAs of each single cell according to the cell tags.

Method for obtaining single-cell mRNA sequence

Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.

Method of constructing sequencing library

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Shenzhen, China

Xiaofang Cheng