Agency for Science, Technology and Research

Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

National University of Singapore

Xiaodi Su

Wolfgang Knoll

Yen Nee Tan

Soo Jin Chua

Hong Yee Low

Yun Zong

Kwok Wei Shah

Shilin Chen

Laura Sutarlie

Ying-Ju Wu

Steven Lukman

Sian Yang Ow

Siu Yee New

Edwin Chong Wing Cheung

Rudolf Robelek

Sean O'Shea

Guangyu Wang

This disclosure relates to a sensor that detects bacteria cells comprising (a) a primary negatively charged, nanoparticulate sensing material; (b) a secondary positively charged, fluorescent sensing material; (c) a housing; and (d) at least one illuminator. This disclosure further relates to a method for detecting bacteria cells.

Nanomaterial-based bacterial sensors

The present invention provides a sensor for sensing nucleic acid-protein interactions, comprising a noble metal nanoparticle (NP), a double stranded nucleic acid molecule capable of binding with a protein in an aqueous solution and a fluorescent conjugated polymer (CP). The present invention also provides a method for sensing nucleic acid-protein interactions with the sensor as defined above.

Nanoparticle sensor for nucleic acid-protein interaction

The present invention refers to a method of determining protein-nucleic acid interaction. The method comprises mixing a protein with a sample comprising a nucleic acid which is suspected to interact with the protein to form a first mixture. The first mixture can be incubated to allow interaction between the protein and nucleic acid. Metallic nanoparticles are added to the first mixture to obtain a second mixture. An electrolyte is added to the first or second mixture to determine the protein-nucleic acid interaction. The present invention also refers to a kit for determining protein-nucleic acid interaction. The kit comprises a protein capable of interacting with a nucleic acid or a nucleic acid capable of interacting with a protein, and at least one type of metallic nanoparticle.

Method for determining protein-nucleic acid interaction

The present invention relates to a method of forming polymer substrate with variable refractive index sensitivity, the method comprising the steps of: (a) contacting a metal-coated patterned mold with a polymer substrate at a temperature sufficient to deform said polymer substrate to thereby deposit a patterned mask of a metal film on the polymer substrate; and (b) etching away portions of said polymer substrate not covered by said patterned mask under conditions to form a region of variable refractive index sensitivity on said polymer substrate.

Method of forming a polymer substrate with variable refractive index sensitivity

There is provided a method for detecting binding of a DNA-binding protein to a target recognition sequence. The method comprises mixing in a reaction buffer a first set of metal nanoparticles, a second set of metal nanoparticles and a DNA-binding protein to form a mixture, and detecting the aggregation state of the mixture of metal nanoparticles. Each set of metal nanoparticles has a conjugated double-stranded DNA molecule having a single-stranded overhang at one end. The single-stranded overhangs of each set of DNA-conjugated metal nanoparticles are complementary to each other such that annealing of the complementary overhangs results in formation of the target recognition sequence that specifically binds the DNA-binding protein. The reaction buffer comprises an ionic species in a concentration sufficient to result in aggregation of the metal nanoparticles upon annealing of the first and second single-stranded overhang.

Methods for detecting DNA-binding proteins

There is disclosed a method and system for detecting a surface plasmon resonance associated with a fluid sample. The method includes the step of providing a piezoelectric substrate having at least two electrodes thereon, wherein at least one of said electrodes is coupled to a fluid sample. A light beam is transmitted toward the fluid sample to induce a oscillation frequency in the piezoelectric substrate. The oscillation frequency from said electrodes is then measured during transmittance of the light to detect the surface plasmon resonance associated with the fluid sample.

Method for detecting surface plasmon resonance

There is provided a resonator sensor useful for detecting polymorphisms and mutations in DNA. The resonator sensor has a capture molecule immobilised on its surface, the capture molecule being either a probe DNA containing a reference sequence, or a mismatch binding molecule, and being capable of forming a probe DNA/target DNA/mismatch binding molecule complex on the surface of the resonator. A method for detecting mutations in a target DNA, including single nucleotide polymorphisms, is also provided.

Device and method of detecting mutations and polymorphisms in DNA

A sensor chip assembly for use in a sensor capable of Surface Plasmon Resonance (SPR) and gravimetric sensing. The assembly comprising a transparent piezoelectric substrate () having a first surface and a second surface opposite to the first surface. The assembly also comprising first and second thin film metal electrodes () respectively provided on the first and second surfaces of the substrate (). The second thin film metal electrode () being position on the second surface of the substrate () such that a light beam is capable of being transmitted through the second surface of the substrate and reflected from the first thin film metal electrode. The assembly also comprising an attenuated total reflection (ATR) coupler () disposed adjacent to the second thin film metal electrode ().

Growing community of inventors

Singapore, Singapore

Xiaodi Su