University of Michigan

Other

University of Houston System

Lc Sciences Lc

Xeotion Corp.

Yu, Peilin

Gao, Xiaolian

Zhu, Qi

Zhou, Xiaochuan

Zhang, Xiaolin

Hong, Ailing

Xiaochuan Zhou

Xiaolian Gao

Eric M Leproust

Hua Zhang

Jean Philippe Pellois

Qin Xiang

Erdogan Gulari

Peilin Yu

Qi Zhu

Xiaolin Zhang

Nijing Sheng

Peillin Yu

Xiaollan Gao

Xialoian Gao

Xiaioian Gao

Nuing Sheng

Ailing Hong

The present invention relates to the field of nucleic acid sequence replication including PCR. Specifically, the present invention relates to methods and compositions for amplifying one or more target sequences from one or more template sequences. In particular, the present invention provides novel primer designs to enhance specificity of PCR reactions. The present invention also provides methods and compositions to perform the selection of specific sequence sections using specific primers and the amplification of all selected sequence sections using a pair of common primers in a single reaction tube.

Highly selective omega primer amplification of nucleic acid sequences

The invention relates to the measurement and profiling of proteins including phosphoproteins from cells in particular the measurement and profiling of phosphoproteins involved in cancer.

Peptide capture and characterization

A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.

Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports

The present invention relates generally to the fields of genomics, synthetic biology and genetic engineering. More particularly, the present invention concerns the methods that enable parallel multiplex ligation and amplification on surface for making assemblies of nucleic acids of various biological applications and for analysis of biological samples such as DNA, RNA, and proteins.

Making nucleic acid sequences in parallel and use

The present invention describes microfluidic devices that provide novel fluidic structures to facilitate the separation of fluids into isolated, pico-liter sized compartments for performing multiplexing chemical and biological reactions. Applications of the novel devices including biomolecule synthesis, polynucleotide amplification, and binding assays are also disclosed.

Fluidic devices and methods for multiplex chemical and biochemical reactions

This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In particular, the present invention provides methods and devices for selectively converting photogenerated reagent precursors to photogenerated reagents comprising a substrate comprising at least one solid surface containing a plurality of isolated reaction sites; and an optical system operably linked to the substrate comprising a light source and a computer-controlled spatial optical modulator to form an irradiation pattern, wherein the irradiation pattern selectively irradiates a plurality of reaction sites without use of a photolithographic mask. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.

Device for chemical and biochemical reactions using photo-generated reagents

This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In particular, the present invention provides methods and devices for generating a photogenerated reaction in solution on a substrate comprising generating a light beam using an optical device system comprising a light source and a computer-controlled spatial optical modulator comprising a digital micromirror device and using the spatial optical modulator to direct light to fluidly isolated reaction sites under conditions such that a photogenerated reaction takes place. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.

This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In some embodiments, the present invention provides methods and devices for generating one or more selected multimers at specific reaction sites on a substrate comprising contacting the substrate with a liquid solution comprising photo-reagent precursors, isolating the reaction sites, selectively irradiating the reaction sites with a digital micromirror device, and contacting the substrate with monomers. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.

An optical device consists of one or more optical waveguides and mechanical light switches . When a light switch is turned on, it extracts light beam from a waveguide core and redirect the light beam into free space, it redirects an incoming light beam from free space and injects the light beam into the waveguide core , or it performs both functions at the same time, depending on specific applications. On and off states of a light switch are achieved by pulling the light switch into a close vicinity of the waveguide core and by pushing the light switch away from the waveguide core , respectively. An interactive fiat-panel display can be built based on this invention. A plurality of parallel channel waveguides forms a display panel. An array of light beams , injected from an array light source , propagates along waveguide cores until reaches a location where a light switch is turned on. At this location, the light switch redirects the light beams towards a viewer. An image is produced when the light switches are turned on sequentially while the light-intensity distribution on the array light source is synchronically updated. The panel display is capable of responding to an input optical signal by detecting an incoming light beam from a light pen . An array of photodetectors is used to identify the location of the incoming light beam on the display panel and a computer is used to execute a corresponding action accordingly.

Growing community of inventors

Houston, TX, United States of America

Xiaochuan Zhou