University of Louisville Research Foundation, Inc.

Xiaoan Fu

Michael H Nantz

Michael Bousamra

Victor Van Berkel

Richard M Higashi

Provided is a non-invasive method of detecting or screening for a cancer in a subject specimen originating in a tissue outside of the lung. The method includes detecting elevated levels of one or more carbonyl-containing volatile organic compounds (VOCs) that are biomarkers for the cancer in exhaled breath from the subject specimen. The method may further include obtaining exhaled breath from the subject specimen; forming adducts of the carbonyl-containing VOCs with a reactive chemical compound; quantifying the adducts of the carbonyl-containing VOCs to establish a subject value for each of the adducts; and comparing each subject value to a threshold healthy specimen value for each of the adducts of the carbonyl-containing VOCs. One or more subject values at quantities greater than threshold healthy specimen values are also useful for screening for the cancer in the subject specimen.

Noninvasive detection of cancer originating in tissue outside of the lung using exhaled breath

A non-invasive method of detecting or screening for lung cancer in a subject specimen is provided. The method includes detecting elevated levels of one or more carbonyl-containing volatile organic compounds (VOCs) that are biomarkers for lung cancer in exhaled breath from the subject specimen. The method may further include obtaining exhaled breath from the subject specimen; forming adducts of the carbonyl-containing VOCs with a reactive chemical compound; quantifying the adducts of the carbonyl-containing VOCs to establish a subject value for each of the adducts; and comparing each subject value to a threshold healthy specimen value for each of the adducts of the carbonyl-containing VOCs. One or more subject values at quantities greater than threshold healthy specimen values are also useful for screening for lung cancer in the subject specimen.

Noninvasive detection of lung cancer using exhaled breath

A chemical pre-concentrator is provided having a support structure, an airflow conduit, and a layer of a reactive chemical compound on a surface of the support structure is used for collecting and pre-concentrating at least one chemical analyte from a dilute sample. A method of concentrating a gaseous sample is provide that includes exposing the chemical pre-concentrator with a dilute gaseous sample that contains at least one chemical analyte; and forming a conjugate of the at least one chemical analyte. A method of diagnosing a disease state in a mammalian patient is provided using the chemical pre-concentrator.

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Louisville, KY, United States of America

Xiaoan Fu