Medarex Inc.

New Jersey Institute of Technology

Bristol-myers Squibb Compnay

E. R. Squibb Sons, Inc.

Xiao-Ping Dai

Kamalesh K Sirkar

Zhengjian Li

Alahari Arunakumari

Jun Tian

Robert Gang Luo

Javier Garcia

Richard P Martel

Haile Ghebramariam

Yanke Xu

Richard Martel

Nan-Xin Qian

Nicholas Abuabsi

Angela Au

The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40&#xd7;10cells/N mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.

Methods for enhanced protein production

Disclosed are methods relating to amplification and expression of a nucleic acid sequence encoding a polypeptide of interest in recombinant cells, and cell lines and polypeptides produced from such methods. The methods disclosed herein permit the amplification of cell lines that express a polypeptide of interest in a relatively short period of time through the use of a bioreactor.

Methods of gene amplification and expression

An apparatus and method for recovering bioproducts from a feed solution. In one embodiment, the apparatus includes a module housing, a membrane means disposed in the housing for filtering the bioproducts from the feed solution wherein a portion of the membrane means is coated with a polymeric coating, and an adsorbent bed disposed in the housing for retaining the bioproducts which permeate through the membrane, wherein the apparatus is adapted to allow fractionation and purification of the retained bioproducts from the bed by elution.

Method and apparatus for isolation and purification of biomolecules

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Flemington, NJ, United States of America

Xiao-Ping Dai