Becton Dickinson and Company Limited

Xiao Mo

Song Shi

Adrien P Malick

John D Mantlo

Susan M Kircher

William B Brasso

Liping Feng

Vanda White

Jon E Salomon

Tuan-Linh Ngoc Nguyen

Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

Identification of microorganisms using MALDI-TOF-MS on-plate extraction

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Ellicott City, MD, United States of America

Xiao Mo