Micro-tech (nanjing) Co., Ltd.

Hitgen, Inc.

Sichuan Provincial People's Hospital

Micro-tech Co., Ltd.

Xiao Hu

Changqing Li

Hongmei Song

Derong Leng

Jin Li

Zhi Tang

Guoqing Zhong

Jinqiao Wan

Dengfeng Dou

Mingqiao Fan

Yan Zhang

Jingchao Feng

Fei Pan

Xing Wang

Huan Xie

Xiaohu Ge

Benyanzi Yang

Peng Lv

Jian Gao

Yun Mu

Shaojun Liu

Caikui Li

Provided is a multifunctional high-frequency electric knife, comprising processing portion, sheath portion, and operating portion. The processing portion is provided with an electrode and a clip component; the electrode is provided with a hollow tubular portion; the electrode can be pushed out or retracted relative to far end of the sheath portion; the clip component can be pushed out or retracted relative to far end of the sheath portion; the sheath portion is disposed at near end of the processing portion and comprises a hollow pull rod and a spring tube; and the operating part is located at near end of the sheath portion and comprises a high-frequency joint connected to the electrode by wire, a component driving, by operating line, the electrode to move, a member driving the hollow pull rod to move, and a liquid inlet allowing liquid to flow to the hollow tubular portion of the electrode.

Multifunctional high-frequency electric knife

The present invention discloses a method for capturing drug targets, comprising the following steps of: (1) preparing row materials, i.e., compounds and DNA or RNA or one party of other specific affinity materials; (2) linking: covalently linking the compounds to the DNA or RNA or the one party of other specific affinity materials, to obtain labeled compounds; (3) transferring: transferring the labeled compounds obtained in the step (2) into cells by a specific gene transfer method; (4) capturing targets: disrupting the cells in the step (3), and then capturing the DNA or RNA in the step (1) by immobilized complementary DNA or RNA, or capturing the one party of affinity materials in the step (1) by the other party of immobilized specific affinity materials, thus to enrich the targets through the covalently linked labeled compounds and the affinity of the targets; (5) identifying targets: dissociating the enriched targets from a stationary phase, deploying the targets by a gel electrophoresis method or by an equivalent separation method, and comparing differential proteins by proteomics thus to identify the potential targets; and (6) determining targets: expressing or purchasing the targets identified in the step (5) by priority, and comparing by interaction of the compounds with those targets one by one to determine the targets for the compounds. By the method of the present invention, compounds with low membrane permeability may be transferred into cells to be bound with the targets in the cells, to realize the target identification closer to the real physiological environment and thus provide more conclusive evidences for the acting mechanism of the compounds to diseases. Additionally, the method for capturing drug targets of the present invention is novel, easy, efficient, and low in cost, and has good application prospects.

Drug target capturing method

Growing community of inventors

Chengdu, China

Xiao Hu