University of California

Water Bear Health Technology (nantong) Co., Ltd.

Xianting Ding

Marcus Aaron Horwitz

Chih-Ming Ho

Daniel L Clemens

Bai-Yu Lee Clemens

Aleidy Marlene Silva Vite

Theodore Kee

Ting Zhang

Haiyang Xie

Ainur Abula

A microfluidic chip comprises: a first unit which has a channel for a cell sample to pass through and is configured to separate circulating tumor cells in the cell sample; a second unit, a front end of which communicates with a tail end of the first unit, and the second unit is configured to capture single cells from the separated circulating tumor cells and subject the captured single cells to closed lysis; and a gel layer which is provided at the second unit. The microfluidic chip is configured to implement the binding of a protein molecule of the single cell with an antibody in the gel layer after the single cell is lysed. A cell separation and western blotting method using the microfluidic chip comprises: lysing circulating tumor cells, capturing, and implementing the binding of a lysate with an antibody. A manufacture method of the microfluidic chip, comprises: manufacturing a first interlayer and a separation unit; manufacturing a second interlayer and pasting the second interlayer on a basal layer, and manufacturing a single-cell capture unit; and bonding the first interlayer with the separation unit and the second interlayer with the single-cell capture unit.

Microfluidic chip and manufacture method thereof, and cell separation and single-cell western blotting method

The present invention is directed to methods of treating tuberculosis by providing a pharmaceutically effective amount of a combination of drug compounds.

Multi-drug therapies for tuberculosis treatment

Growing community of inventors

Shanghai, China

Xianting Ding