Gen-probe, Inc.

Xianqun Wang

Sangeetha Vijaysri Nair

Susan K Yamagata

James M Carrick

Michael J Gilly

Jennifer Jill Bungo

Tongjia Yin

Michael Hadjisavas

Steven T Brentano

Damon Kittredge Getman

Somalee Datta

Angela S Hudson

Jimmykim Pham

Steve Brentano

Mingjie Wang

Brett Bowman

Caroline Clark

Angela Sebring Hudson

Method and system for quantifying target nucleic acids using real-time amplification and internal calibration adjustment. The invention employs dual reference calibration curves for approximating a complete calibration curve from only a single adjustment calibrator amplified on the instrument that is to be calibrated.

Single-point calibration of a nucleic acid analyzer

Disclosed are methods for diagnosing Bacterial Vaginosis in a subject comprising performing an assay for the detection of any one or more ofsp., Atopobium vaginae, andin a subject sample. Also disclosed are compositions and methods for detectingsp., Atopobium vaginae, and/ornucleic acid in a sample.

Compositions and methods for detecting bacterial nucleic acid and diagnosing bacterial vaginosis

Presented are methods, systems, and software products useful for determining the concentration of an analyte in a fluid specimen used to produce a dried sample, where the dried sample serves as a source of the analyte in a detection and quantification procedure. Particularly illustrated is the use of dried blood spots for quantifying a polynucleotide analyte.

Method of quantifying polynucleotide analytes from dried samples

Quantification of polynucleotide analytes from dried samples

The invention provides methods of determining a consensus sequence from multiple raw sequencing reads of a nucleic acid target. The nucleic acid target includes an anchor segment of known sequence and an adjacent segment of unknown sequence. The anchor segment provides a means to assess the quality of a raw target sequencing read. Raw target sequencing reads meeting or exceeding a threshold are assigned to an accepted class. The consensus sequence of the adjacent segment can be determined from raw target sequencing reads in the accepted class. Successive polling steps determine successive consensus nucleobases in a nascent sequence of the adjacent segment. Raw target sequencing reads can be removed or reintroduced from the accepted class depending on their correspondence to the most recently determined consensus nucleobase and/or the nascent sequence.

Algorithms for sequence determinations

Method and system for quantifying target nucleic acids using real-time amplification and internal calibration adjustment. The approach employs a single fixed data point in combination with a single adjustment calibrator amplified on the instrument that is to be calibrated for approximating a complete calibration curve.

Calibration method, apparatus and computer program product

Method of calibrating a nucleic acid analyzer

Disclosed are methods for detecting a minority genotype of a target nucleic acid. The disclosed method generally includes the steps of (a) deep sequencing at least a portion of the target nucleic acid; (b) using the deep sequencing results of (a) to detect the presence of variant nucleobases at one or more nucleotide reference positions within the target nucleic acid; (c) using the variant detection results generated in step (b) to perform a statistical analysis of whether the variants are significant; and (d) using the variant detection and variant significance results generated in steps (b) and (c) to perform a statistical analysis of whether a subset of sequences together exhibit a common set of significant variants.

Method for detecting a minority genotype

Dual reference calibration method and system for quantifying polynucleotides

Growing community of inventors

San Marcos, CA, United States of America

Xianqun Wang