Jiangsu Academy of Agricultural Sciences

Jiangsu Academy of Agricultural Sciences Institute of Food Safety and Monitoring Technology

Xianjin Liu

Xiao Zhang

Yuan Liu

Chongxin Xu

Yajing Xie

Cunzheng Zhang

Xiangyang Yu

Donglan Wang

Xin He

Meijing Gao

Aihua Wu

Yanyan Zhao

Jianfeng Zhong

The present invention provides a human-derived molecularly modified insecticidal protein, and a preparation method and application thereof, and belongs to the field of genetic engineering and biological control. The present invention provides a human-derived molecularly modified insecticidal protein, and the amino acid sequence of the insecticidal protein CCL-CCL_scFv is shown as SEQ ID No. 1. The insecticidal protein CCL-CCL_scFv shows significantly higher affinity with midgut BBMV ofthan Cry1Ab toxin, competes with Cry1Ab and Cry1Ac toxins for binding the midgut BBMV of, and is a mimic of Cry1Ab and Cry1Ac toxins. Throughindoor insecticidal biological activity assay, the insecticidal protein shows a good insecticidal effect, and can effectively replace Cry1Ac or Cry1Ab toxin for biological control of insect pest.

Human-derived insecticidal bt cry toxin mimetic, and design method and application thereof

Provided are a human-derived insect-resistant gene having a nucleotide sequence represented by SEQ ID NO.1, and an anti-Cry1B toxin idiotype single-chain antibody encoded by said human-derived insect-resistant gene and having an amino acid sequence represented by SEQ ID NO.2. The idiotype single-chain antibody is a &#x3b2;-type and has insecticidal activity, and after expression by the prokaryotic system, the primary culture thereof has binding activity tomidgut peritrophic membrane specific receptor BBMV.

Human-derived insect-resistant gene and anti-CRY1B toxin idiotype single-chain antibody encoded thereby and application thereof

An anthropogenic pest-resistant gene having a nucleotide sequence as represented by SEQ IN NO. 1, and, an anti-Cry1Ab toxin idiotypic single-chain antibody encoded by the anthropogenic pest-resistant gene and having a nucleotide sequence as represented by SEQ IN NO. 2.

Human-derived insect-resistant gene and anti-Cry1B toxin idiotype single-chain antibody encoded thereby and application thereof

An anthropogenic insect-resistant gene having a nucleotide sequence represented by SEQ ID NO.1, and a Cry1C toxin idiotype single-chain antibody encoded by said anthropogenic insect-resistant gene and having an amino acid sequence represented by SEQ ID NO.2; the antibody is a &#x3b2;-type and has insecticidal activity, and after expression by the prokaryotic system, the primary culture thereof has binding activity tomidgut peritrophic membrane specific receptor BBMV; the &#x3b2;-type Cry1C toxin idiotype single-chain antibody of the present invention is obtained without animal immunization, has a short preparation period and small amino acid sequence, and is suitable for large-scale in vitro production. The present invention is an entirely new insect-resistant gene resource, and has significant implications for decreasing the various safety risks associated with the widescale use of existing Bt toxins, substituting Bt toxins in the biocontrol of agricultural pests, and reducing the use of pesticides.

Anthropogenic insect-resistant gene and Cry1C toxin idiotype single-chain antibody encoded thereby and application thereof

The present invention discloses a human-derived insecticidal gene and insecticidal peptide encoded by the same and application thereof. The nucleotide sequence of the human-derived insecticidal gene is as represented by SEQ ID NO.1. The amino acid sequence of the insecticidal peptide encoded by this gene is as represented by SEQ ID NO.2. The insecticidal peptide may be expressed through prokaryotic system. The primary culture has binding activity tomidgut peritrophic membrane specific receptor BBMV. It is obtained without animal immunization and has a short production cycle and a small amino acid sequence. It is suitable for in vitro mass production and may lower the safety risks resulting from wide use of existing Bt toxins and even might substitute Bt to biologically control agricultural pests in the future. It has important scientific and practical significance to reducing the use of insecticides.

Human-derived insecticidal gene and insecticidal peptide encoded thereby and application thereof

Growing community of inventors

Xuanwu Nanjing, China

Xianjin Liu