Hubei University

Wuhan Polyploid Biotechnology Co., Ltd

Xianhua Zhang

Zhaojian Song

Yuchi He

Detian Cai

Yuhua Liu

Wei Wang

A breeding method of a polyploid rice photo-thermo-sensitive genetic male sterile line includes determining a diploid rice line with photo-thermo-sensitive genetic male sterility or PMeS characteristic as a parent; carrying out hybridization on a diploid photo-thermo-sensitive genetic male sterile line and a diploid PMeS gene line, carrying out doubling culture on a young ear of a hybrid plant into a hybrid tetraploid; back-crossing the hybrid tetraploid with a tetraploid photo-thermo-sensitive genetic male sterile line; selecting a tetraploid male sterile plants from the back-crossed progeny, self-crossing during a low-temperature and short-day fertile period, and then carrying out composite hybridization with another tetraploid rice line having PMeS gene; selecting tetraploid male sterile plants, and detecting the stability of tetraploid male sterile plants after multiple generations of continuous self-crossing; and determining the stable and consistent tetraploid rice sterile line as the polyploid rice photo-thermo-sensitive genetic male sterile line, named as PSXXX.

Polyploid rice photo-thermo-sensitive genetic male sterile line and breeding method thereof

A breeding method of a polyploid rice two-line restorer line includes determining hybrid parents for breeding the restorer line; breeding a restorer line of an indica sterile/japonica restorer type, or breeding a restorer line of a japonica sterile/indica restorer type, and carrying out backcrossing or composite hybridization after hybridization of the parents; selecting a single plant that meets the breeding goal, carrying out composite hybridization again and carrying out preliminary molecular marker detection; comparing, and selecting a single plant with good traits for continuous selfing until the line is basically stable; selecting a stable line with good traits and detection molecular markers; carrying out test-crossing on the preferred line as a male parent with different types of polyploid sterile lines; and selecting a good hybrid combination and a restorer line thereof.

Polyploid rice two-line restorer line and breeding method thereof

A method for breeding polyploid two-line hybrid rice includes determining a tetraploid rice photo thermosensitive genic male sterile line with the gene characteristic of PMeS (polyploid meiosis stability) and a tetraploid rice restoring line with the gene characteristic of PMeS; hybridizing and matching by indica sterile/restoring orsterile/indica restoring hybrid combination; preparing a tetraploid rice hybrid by adopting a tetraploid rice photo thermosensitive genic male sterile line and a tetraploid rice restoring line; and breeding a stable tetraploid rice hybrid combination which is determined as the polyploid two-line hybrid rice combination. The breeding method disclosed by the present invention utilizes the strong heterosis of polyploid rice, and transforms the existing diploid heterosis into the heterosis of polyploid two-line hybrid rice; and by adopting the method disclosed by the present invention, a new polyploid two-line hybrid rice variety with large ears, large grains and high yield can be bred.

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Hubei, China

Xianhua Zhang