Wisconsin Alumni Research Foundation

Xiangyang Liu

Aseem Zoe Ansari

Jose Arcadio Rodriguez-Martinez

A cyanuric acid-responsive biosensor includes a host strain or cell-free system including a first and a second expression cassette; the first expression cassette including, in operable communication, a constitutive promoter and a gene encoding an AtzR cyanuric acid-responsive transcription factor; and the second expression cassette including, in operable communication, a promoter regulated by the cyanuric acid-responsive transcription factor and a gene encoding a reporter protein, wherein the first expression cassette expresses the AtzR cyanuric acid-responsive transcription factor, and, in the presence of cyanuric acid, the AtzR cyanuric acid-responsive transcription factor drives expression of the reporter protein.

Sensor for cyanuric acid detection

Described herein is a method for identifying synthetic inducible promoters that have specified induction and/or repression for DNA binding proteins such as an allosteric transcription factor and an inducer molecule. The method includes an in vitro selection from an unselected polynucleotide library comprising a plurality of random degeneracies, and an in vivo selection to produce an induced promoter library. Produced is an induction table, which allows the selection of a promoter with specific induction and/or repression properties. Also included are biosensors containing the synthetic inducible promoters.

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Madison, WI, United States of America

Xiangyang Liu