Arizona State University

Xiangxing Kong

Deirdre Meldrum

Yanqing Tian

Fengyu Su

Liqiang Zhang

Laimonas Kelbauskas

Hong Wang

Ye Hu

Ganquan Song

Wacey Teller

Meryl Rodrigues

Qingbo Shu

Tanxi Cai

Provided herein are methods for detecting and identifying disease-specific biomarkers, such as target antigens associated with infection.

Detection of antigens

A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells.

Device and method for high-throughput multiparameter measurements in one or more live and fixed cells

Fluorescent pH sensors are provided. The fluorescent pH sensor comprises a copolymer for sensing pH and a polymerized form of N-(2-hydroxypropyl)methacrylamide (HPMA) or 2-hydroxyethyl methacrylate (HEMA). The probe for sensing pH has formula (I): wherein Rand Rare as defined herein. The fluorescent pH sensors may be used in determining the pH of a sample and detecting extracellular pH in a sample. Methods for preparing the fluorescent pH sensors and the probe for sensing pH are also provided.

Fluorescent pH sensors and methods of preparing them

Mitochondria-targeting potassium sensors and method(s) for making such sensors. The sensor shows a response to potassium and displays a 130-fold dynamic range of fluorescence intensity and high brightness. The sensors response to potassium concentrations was demonstrated to be unaffected by cellular pH value and/or concentrations of other ions. The sensors can be used for monitoring the mitochondrial potassium efflux/influx.

Mitochondria-targeting fluorescent potassium+ sensor and method of making the same

Growing community of inventors

Tempe, AZ, United States of America

Xiangxing Kong