Shenzhen Mindray Bio-medical Electronics Co., Ltd.

Xiangping Meng

Bing Liu

Mulong Liu

Wenjuan Xu

Yanmei Qiao

A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40&#xb0; C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.

Reagents and methods for classifying leukocytes

A reagent for four-part differentiation of white blood cells is provided. In one embodiment the reagent has an osmolality below 50 mOsm/kg HO. A method for differentiating white blood cells using the reagent is also provided. The disclosure provides for a rapid lysis of red blood cells and four-part differentiation of white blood cells. The reagent may be simple in components and a surfactant is not necessary, but optional. A wide range of pH values may be suitable for the reagent.

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Shenzhen, China

Xiangping Meng