Institute of Genetics and Developmental Biology, Cas

Xiangbing Meng

Bing Wang

Guifu Liu

Jiayang Li

Yanhui Jing

Hong Yu

Mingjiang Chen

Huihui Liu

The disclosure discloses a method for preparing a multisubunit SCF E3 ligase with a fusion protein through in vitro reconstitution, and a use of the multisubunit SCF E3 ligase. The method for preparing a multisubunit SCF E3 ligase with a fusion protein through in vitro reconstitution disclosed by the disclosure includes: subjecting an engineered SKP1-Cullin1-RBX1 fusion protein (referred to as eSCR) to a reaction with an F-box protein in a reaction system to obtain the multisubunit SCF E3 ligase, where the eSCR fusion protein has an amino acid sequence from position 10 to position 1062 of SEQ ID NO: 2. Experimental results show that different multisubunit SCF E3 ligases are successfully prepared with the eSCR fusion protein through in vitro reconstitution in the disclosure; the reconstituted multisubunit E3 ligase has biological activity.

Method for preparing multisubunit SCF E3 ligase with fusion protein through in vitro reconstitution, and use of multisubunit SCF E3 ligase

Growing community of inventors

Beijing, China

Xiangbing Meng