Advion Biosciences, Inc.

Advion Bioscience, Inc.

Advion Inc.

Advion Biosystems, Inc.

Xian Huang

Gary A Schultz

Thomas N Corso

Simon Prosser

Sheng Zhang

Simon J Prosser

A microfabricated silicon chip with a separation material, such as in situ prepared porous polymer monoliths in its microchannels is disclosed. The polymer monoliths are liquid-permeable and serve as microcolumns for liquid chromatography, which are prepared by in situ radical polymerization of a mixture containing vinyl monomers and solvents (porogen) in the microchannels. A method and system are disclosed to generate one or more electrospray plumes from one or more nozzles that provide an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analyses contained within the fluid. A plurality of electrospray devices can be used in the form of an array of miniaturized separate electrospray devices for the purpose of generating multiple electrospray plumes from multiple nozzles for the same fluid for analysis. This invention dramatically increases the sensitivity of microchip electrospray devices compared to prior disclosed systems and methods. The silicon chip having the packed microchannels disclosed herein finds application in coupling with mass spectrometry for sample analysis. Also disclosed is a separation block having multiple through-substrate channels filled with a separation material such as polymer monolith which can be stacked in multiple blocks for sequential two-dimensional chromatographic separation and integrated with the electrospray device.

Separation media, multiple electrospray nozzle system and method

A process is provided for modifying a porous polystyrene monolith to render its internal pore surfaces grafted with alkyl groups, preferably of at least four carbon atoms, which includes the step of alkylating the monolith with a uniform liquid solution containing a Friedel-Crafts catalyst and an alkyl halide. A surface-modified monolith produced thereby is an efficient separation medium in reversed-phase liquid chromatography for both small and large biomolecules.

Surface modification of a porous polymer monolith and products therefrom

A microchip-based electrospray ionization device and column with affinity adsorbents is disclosed. The invention includes a microchip array and a capillary tube or alone or attached in combination. At least a portion of the device or column has immobilized affinity adsorbents. Methods for using the device are provided as well for affinity capture of biomolecules to meet the needs for the modem life sciences such as proteomics and drug discover.

Microchip electrospray device and column with affinity adsorbents and use of the same

A microchip-based electrospray ionization device and column with affinity adsorbents is disclosed. The invention includes a microchip array and a capillary tube or alone or attached in combination. At least a portion of the device or column has immobilized affinity adsorbents. Methods for using the device are provided as well for affinity capture of biomolecules to meet the needs for the modern life sciences such as proteomics and drug discover.

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Ithaca, NY, United States of America

Xian Huang