Mgi Tech Co., Ltd.

Bgi Shenzhen

Huawei Technologies Co., Limited

Xia Zhao

Hui Fu Jiang

Yuan Jiang

Radoje Drmanac

Wenwei Zhang

Chunyu Geng

Kai Tian

Qiaoling Li

Huaiqian Xu

Bo Wang

Li Chen

Jing Guo

Andrei Alexeev

Lingyu He

Xiaojun Ji

Yaqiao Li

Shengmao Liu

Dong Li

Jing Zhao

Ao Chen

Tong Wang

Fang Chen

Chao Dong

Rongrong Guo

Wen-wei Zhang

Shujin Fu

Evan Hurowitz

Ruoying Chen

Xingye Cui

Yang Xi

Hui Jiang

Xiaoshan Su

Fanzi Wu

Kai Kang

Yun Xia

Hanjie Shen

Jie Ruan

Yuzhong Sun

Ying Song

A method for analyzing droplets based on images: obtaining a working dye channel image and a reference dye channel image corresponding to a droplet system (S); separately performing a preset image processing operation on the working dye channel image and the reference dye channel image (S); using the working dye channel image or the reference dye channel image as a target image (S), and determining a region in which each droplet is located from within the target image (S); and on the basis of the determined region in which each droplet is located, analyzing the droplet system (S). The method can effectively improve the accuracy of droplet analysis. Further provided are a computer device and a storage medium.

Method for analyzing droplets based on images, computer device and storage medium

A separation and collection device for cells and biomolecules includes an annular tube and several magnetic beads configured to identify and bind with different cells or molecules in the biological sample. The annular tube defines a through hole for the biological sample to exit. Sizes of different groups of magnetic beads are different. An aperture size of a through hole is changeable such that the plurality of groups of magnetic beads can flow through the through hole in a certain order. A testing system including the separation and collection device is further disclosed.

Separation and collection device for cells and biomolecules, and testing system

Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

Bubble-shaped adaptor element and method of constructing sequencing library with bubble-shaped adaptor element

Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.

Vesicular adaptor and uses thereof in nucleic acid library construction and sequencing

Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.

Vesicular linker and uses thereof in nucleic acid library construction and sequencing

Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3&#x2032; end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3&#x2032; end thereof can be in a complementary pairing with the 5&#x2032; end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.

Linker element and method of using same to construct sequencing library

A method and reagent for constructing a nucleic acid double-linker single-strand cyclic library. The method comprises: breaking a nucleic acid into nucleic acid fragments; connecting a first linker sequence; producing by amplification a first product provided with the first linker sequence at either end, where a U nucleobase site is provided on primer sequences and a nicking enzyme recognition sequence is either provided or not provided on same, and a first affinity tag is provided on one of the primer sequences; using USER enzyme to cleave the first product; cyclizing the cleaved first product; treating the cyclization product with either a phosphatase or a nicking enzyme; using a solid-phase vector for combination with a cyclized molecule; performing a restrictive gap translation reaction; removing by digestion any portion that did not undergo the restrictive gap translation reaction; connecting a second linker sequence; producing by amplification a second product provided with the second linker sequence at either end; denaturing the second product, and cyclizing a single-strand nucleic acid molecule. The method allows an increase in the length of library insert fragments, a simplified library construction process, reduced library construction time, and reduced library construction costs.

Method and reagent for constructing nucleic acid double-linker single-strand cyclical library

Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Method for constructing nucleic acid single-stranded cyclic library and reagents thereof

Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3'-terminal and the 5&#x2032;-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3&#x2032;-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.

Method of constructing sequencing library with bubble-shaped adaptor element

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Shenzhen, China

Xia Zhao