Petrochina Company Limited

Chinese Academy of Agricultural Sciences

Xia Li

Chunhui Zhang

Zhonghua Liu

Chao Yuan

Hongru Zhang

Jixin Deng

Lianteng Song

Xiangzhi Cheng

Haitao Zhang

Wei Jia

Xiaoming Yang

Song Hu

Xiaojie Qin

Yujie Guo

Hongjun Xu

Baoyin Xue

Peiyuan Zhang

Cancan Zhou

Chaoliu Li

Changxi Li

Qiangfu Kong

The present disclosure relates to a gene recombinant vector of a collagenase, comprising a collagenase gene, wherein an amino acid sequence of a collagenase encoded by the collagenase gene is shown in SEQ ID NO. 1; moreover, a genetically engineered strain of the collagenase and a preparation method of the collagenase are also disclosed; and the collagenase prepared according to the invention is capable of degrading a bone collagen, and improving a yield of a low-molecular-weight bone collagen peptide.

Gene recombinant vector, genetically engineered strain and preparation method of collagenase

A method for obtaining a conversion relationship between dynamic and static elastic parameters includes: Step S, acquiring horizontal cores at different depths of the destination formation; Step S, measuring the dynamic elastic parameters of the horizontal core under different pressures; Step S, measuring the static elastic parameters of the horizontal core under different pressures; Step S, measuring the clay content of the horizontal core; Step Sestablishing a function relationship of the ratio between the dynamic and static elastic parameters with the formation pressure and clay content; and completing the conversion between the dynamic and static elastic parameters. The technical solution provided by the present invention takes full account of the influence of the formation stress and the clay content on the conversion rule of dynamic and static elastic parameters and is of great significance for improving the logging evaluation accuracy of rock mechanical parameters.

Method for obtaining conversion relationship between dynamic and static elastic parameters

The invention discloses a liquefaction device of hard bone, comprising: outer cavity having upper end detachably sealed with upper cover, and lower end openable/closable sealed with lower sealing cover, first liquefaction cavity coaxially slidably disposed in outer cavity, stainless steel cage disposed coaxially in first liquefaction cavity, second liquefaction cavity fixed to outer sidewall of outer cavity such that lower end portion of outer cavity is located in second liquefaction cavity. The invention also provides a method for co-production of bone collagen polypeptide and ultrafine bone powder based on liquefaction device, including: selecting hard bones, crushing; performing first-stage and second-stage liquefaction to obtain liquid phase and solid phase; centrifuging, concentrating, drying liquid phase to obtain bone collagen polypeptide; drying, coarsely and superfine pulverizing solid phase to obtain ultrafine bone powder. The invention has effects of simplifying process, improving production efficiency, and reducing production and equipment investments.

Liquefaction device of hard bone and method for co-production of bone collagen polypeptide and ultrafine bone powder

The present invention proposes a method and apparatus of determining stiffness coefficients of formation, wherein the method comprising: setting up a relation of stiffness coefficients of formation Cand C, C, Cbased on stiffness coefficients of a formation core sample; computing clay content of formation along depth continuously based on formation logging information; computing the stiffness coefficient of formation Calong depth continuously based on a P-wave velocity and a volume density of the formation; computing the stiffness coefficient of formation Calong depth continuously based on a S-wave velocity and a volume density of the formation; and then computing the stiffness coefficients of formation Cand Calong depth continuously based on the calculations above, the relation of stiffness coefficients of formation Cand C, C, C, and a relation of an anisotropy coefficient of the P-wave of the formation and its clay content or a relation of an anisotropy coefficient of the S-wave of the formation and its clay content. The present invention does not need to calculate stiffness coefficients by using a horizontal S-wave velocity inversed from the extracted stoneley waves, and avoids an extraction process of horizontal S-wave, such that the calculation is simple and effective.

Method and apparatus of determining stiffness coefficients of formation

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Beijing, China

Xia Li