Massachusetts Institute of Technology

Harvard College

The Broad Institute, Inc.

Xi Chen

Fei Chen

Omar O Abudayyeh

Jonathan S Gootenberg

Kaiyi Jiang

Rohan Neil Krajeski

Yifan Zhang

Jeremy G Koob

RNA editing tools for use in systems designed to measure RNA in vivo and manipulate specific cell types are disclosed herein. An RNA sensor system comprising a) a single-stranded RNA (ssRNA) sensor comprising a stop codon and a payload; optionally wherein the ssRNA sensor further comprises a normalizing gene; and b) an adenosine deaminase acting on RNA (ADAR) deaminase; wherein the sensor is capable of binding to a ssRNA target to form a double-stranded RNA (dsRNA) duplex that becomes a substrate for the ADAR deaminase; wherein the substrate comprises a mispairing within the stop codon; and wherein the mispairing is editable by the ADAR deaminase, which editing can effectively remove the stop codon so as to enable translation and expression of the payload. A method of quantifying ribonucleic acid (RNA) levels using the RNA sensor system is also disclosed.

Deaminase-based RNA sensors

Growing community of inventors

Roxbury Crossing, MA, United States of America

Xi Chen