Sanofi

National Institutes of Health, a Component of the US Dept. of Health & Human Services

Wulf Dirk Leuschner

Gary J Nabel

Peter D Kwong

John R Mascola

Huawei Qiu

Jochen Beninga

Joachim Wink

Tongqing Zhou

Ercole Rao

Christian Lange

Christian Beil

Edward Seung

Jochen Kruip

Mark Connors

Zhi-Yong Yang

Luigi Toti

Young Do Kwon

Carsten Corvey

Lan Wu

Ronnie Rong Wei

Ling Xu

Rolf Müller

Mark Broenstrup

Joerg Birkenfeld

Jinghe Huang

Johann Gassenhuber

Silke Wenzel

Marion Schneider

Amarendra Pegu

Mangaiarkarasi Asokan

Werner Dittrich, I

Claudia Koenig

Tina Binz

Carsten Volz

Ronnie Wei

Nicole A Doria-rose

Richard A Koup

Caudia Koenig

Rolf Mueller

The disclosure provides trispecific and/or trivalent binding proteins comprising four polypeptide chains that form three antigen binding sites that specifically bind one or more target proteins, wherein a first pair of polypeptides forming the binding protein possess dual variable domains having a cross-over orientation and wherein a second pair of polypeptides forming the binding protein possess a single variable domain. The disclosure also provides methods for making trispecific and/or trivalent binding proteins and uses of such binding proteins.

Trispecific and/or trivalent binding proteins

The present invention relates to a method for producing a fusion polynucleotide encoding a polypeptide of interest. The method comprises the steps of providing a first polynucleotide and a second polynucleotide, and contacting said first polynucleotide and second polynucleotide with a type IIs restriction endonuclease and a ligase under conditions that allow for the cleavage of the first polynucleotide and second polynucleotide by said type IIs restriction endonuclease and the ligation of the resulting cleavage products, thereby producing the fusion polynucleotide encoding the polypeptide of interest. The first polynucleotide comprises the 5' portion of an intron, and the second polynucleotide comprises the 3&#x2032; portion of an intron. Further envisaged by the present invention is a polynucleotide encoding a polypeptide of interest, which, when transcribed in a eukaryotic host cell, is transcribed into a transcript comprising at least five introns which are heterologous to said polynucleotide.

Intron-based universal cloning methods and compositions

Provided herein are bivalent, bispecific binding proteins that specifically bind to two different HIV-1 Env protein epitopes.

Bivalent, bispecific binding proteins for prevention or treatment of HIV infection

The present invention refers to the gene cluster and genes comprised by the gene cluster which are involved in the biosynthesis of griselimycin and methylgriselimycin and to the use of the gene cluster, genes comprised thereby and proteins encoded thereby for the production of antibiotic agents.

Gene cluster for biosynthesis of griselimycin and methylgriselimycin

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Frankfurt am Main, Germany

Wulf Dirk Leuschner