Sanofi

The United States of America, As Represented by the Secretary, Department of Health and Human Services

Wulf Dirk Leuschner

Gary J Nabel

Peter D Kwong

John R Mascola

Huawei Qiu

Lan Wu

Joachim Wink

Tongqing Zhou

Ercole Rao

Christian Lange

Zhi-Yong Yang

Edward Seung

Jochen Kruip

Mark Connors

Jochen Beninga

Carsten Corvey

Young Do Kwon

Christian Beil

Ronnie Rong Wei

Ling Xu

Luigi Toti

Rolf Müller

Mark Broenstrup

Joerg Birkenfeld

Jinghe Huang

Silke Wenzel

Johann Gassenhuber

Marion Schneider

Amarendra Pegu

Werner Dittrich, I

Mangaiarkarasi Asokan

Richard A Koup

Ronnie Wei

Claudia Koenig

Tina Binz

Carsten Volz

Nicole A Doria-rose

Caudia Koenig

Rolf Mueller

The disclosure provides trispecific and/or trivalent binding proteins comprising four polypeptide chains that form three antigen binding sites that specifically bind one or more target proteins, wherein a first pair of polypeptides forming the binding protein possess dual variable domains having a cross-over orientation and wherein a second pair of polypeptides forming the binding protein possess a single variable domain. The disclosure also provides methods for making trispecific and/or trivalent binding proteins and uses of such binding proteins.

Trispecific and/or trivalent binding proteins

The present invention relates to a method for producing a fusion polynucleotide encoding a polypeptide of interest. The method comprises the steps of providing a first polynucleotide and a second polynucleotide, and contacting said first polynucleotide and second polynucleotide with a type IIs restriction endonuclease and a ligase under conditions that allow for the cleavage of the first polynucleotide and second polynucleotide by said type IIs restriction endonuclease and the ligation of the resulting cleavage products, thereby producing the fusion polynucleotide encoding the polypeptide of interest. The first polynucleotide comprises the 5' portion of an intron, and the second polynucleotide comprises the 3&#x2032; portion of an intron. Further envisaged by the present invention is a polynucleotide encoding a polypeptide of interest, which, when transcribed in a eukaryotic host cell, is transcribed into a transcript comprising at least five introns which are heterologous to said polynucleotide.

Intron-based universal cloning methods and compositions

Provided herein are bivalent, bispecific binding proteins that specifically bind to two different HIV-1 Env protein epitopes.

Bivalent, bispecific binding proteins for prevention or treatment of HIV infection

The present invention refers to the gene cluster and genes comprised by the gene cluster which are involved in the biosynthesis of griselimycin and methylgriselimycin and to the use of the gene cluster, genes comprised thereby and proteins encoded thereby for the production of antibiotic agents.

Gene cluster for biosynthesis of griselimycin and methylgriselimycin

Growing community of inventors

Frankfurt am Main, Germany

Wulf Dirk Leuschner