Fuso Pharmaceutical Industries, Ltd.

Osaka Prefecture University Public Corporation

Osaka Prefecture University

Worada Samosorunsuk

Masahiro Asakura

Shinji Yamasaki

An objective of the present invention is to provide the cytolethal distending toxin (CDT) ofand polynucleotides encoding it, and novel methods for detection ofusing the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) ofbacteria, and detected the cdt genes of a-like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in, and, but not by multiplex PCR that can specifically detect the cdtA, cdtB, and cdtC genes of the three bacterial species. The bacterial strain was identified asby 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.

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Osaka, Japan

Worada Samosorunsuk