Korea Research Institute of Chemical Technology

Woo-Keun Kim

Seokjoo Yoon

Jieon Lee

Sangwoo Lee

Chul Min Park

Bomi Shin

The present invention relates to a composition for temperature sensors including graphene oxide, DNAzyme and PNA, a temperature sensing method and a kit using the same. In the present invention, when the DNAzyme/PNA duplex is dissociated at a certain temperature, graphene oxide adsorbs PNA with excellent selectivity and irreversibility, enabling recall of temperature, to permit delayed color development in the time of need. Also, the target temperature can be easily and quickly detected with the naked eye through the color change of the colorimetric reagent, and the thermosensor is technically convenient and easy to apply, so it can be used in various biological applications. Moreover, it can be used as a barcode (on/off) system using a combination of PNA probes with various lengths, and thus can be broadly applied to sensing a diverse range of temperatures.

Composition for temperature sensors including graphene oxide, DNAzyme and PNA and temperature sensing method using the same

The present invention provides a complex of LNA probe and graphene oxide, and a nucleic acid detection method using the same. In the present invention, LNA-containing molecular beacon is conjugated through covalent bonding with graphene oxide, a single strand of the molecular beacon binds to a target nucleic acid to form a complex, and the complex is separated from graphene oxide to induce a fluorescence signal. The molecular beacon and graphene oxide can be covalently bonded to minimize non-specific signals, and a LNA-added molecular beacon is designed in a double strand to detect a very low concentration of target nucleic acid with high sensitivity, as well as a fluorescent signal, and the multiple target nucleic acids can be detected simultaneously through diversification of the fluorescent signal to enable easy and accurate detection of a nucleic acid biomarker whose specific expression level is specifically changed according to diseases and disease progression.

Complex of LNA probe and graphene oxide and nucleic acid detection method using same

The present invention relates to a composition for detecting nucleic acid comprising duplex molecular beacon and graphene oxide and a colorimetric signal enhancement method of detecting nucleic acid using the same. According to the composition, kit and method for detecting nucleic acid of the present invention, a complex can be formed by adsorbing a single strand having a DNAzyme sequence dissociated from the conjugate of a duplex molecular beacon and a target nucleic acid to the graphene oxide surface, and separated, and a colorimetric signal amplified therefrom can be induced, so that a very low concentration of target nucleic acid can be detected with high efficiency and the target nucleic acid can be detected quickly and easily in seconds. Therefore, a new colorimetric target nucleic acid detection system capable of point of care testing (POCT) can be provided.

Composition for detecting nucleic acid and colorimetric signal enhancement method of detecting nucleotide using thereof

The present invention relates to a bis phenyl hexa triene derivative, a preparation method thereof and a composition for skin whitening comprising the same as an active ingredient. The said derivative inhibits the expression of tyrosinase, TRP 1 (tyrosinase-related protein 1), TRP 2 (tyrosinase-related protein 2) or MITF (microphthalmia-associated transcription factor) gene which produces a precursor necessary for the generation of melanin, suppresses the activity of tyrosinase, eventually inhibits the biosynthesis of melanin, is safe due to low cytotoxicity, and has less side effects and excellent antioxidative effect, so that it can be effectively used as a composition for skin whitening.

Bis phenyl hexa trien derivatives, preparation method thereof, and composition for skin whitening containing the same as an active ingredient

An apparatus for observing a fine object is disclosed. The observation apparatus includes: a camera configured to capture each of a plurality of fine objects contained in at least some of a plurality of partitioned wells of a plate; a drive unit configured to allow the camera to relatively move with respect to the plate in such a manner that the camera scans the plate; and a controller configured to control the drive unit in such a manner that the camera captures only at least some wells having the plurality of fine objects from among the plurality of wells. The controller determines a shortest movement route of the camera on the basis of a position of at least some wells having the plurality of fine objects, and controls the drive unit in such a manner that the camera moves in the determined shortest movement route.

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Daejeon, South Korea

Woo-Keun Kim