Sk Innovation Co., Ltd.

Sk Energy Co., Ltd.

Woo Chan Park

Jae Yeon Park

Joong Min Park

In Ho Cho

Min Su Koo

Seung Yeop Lee

Sin Young Kang

Sang Min Lee

Dong Hyun Kim

Taewan Kim

Jae Wook Ryu

Laura Ruohonen

Marja Ilmen

Yeon Hwa La

Young Bin Seo

Outi Koivistoinen

Merja Oja

Kwang Kuk Cho

Joon Sik Lee

Andrew Conley

Paula Jouhten

Eun Hee Park

Jayoung Ryu

Young Hwan Chu

The present invention relates to a process of converting carbon dioxide using a combination of a carbon dioxide mineralization and the metabolism of sulfur-oxidizing microorganisms. According to the process, a carbonate produced in the carbon dioxide mineralization reaction can be converted to a useful substance without supplying an external additional energy source (light, electrical energy, etc.) and mineral resources (metal ions). In addition, the process can be continuously performed by recycling metal ions necessary for the carbon dioxide mineralization reaction.

Process of converting carbon dioxide using combination of carbon dioxide mineralization process and metabolism of sulfur-oxidizing microorganisms

Provided are a recombinant yeast producing 3-hydroxypropionic acid (3-HP) and a method for producing 3-HP using the same, more particularly, a recombinant yeast producing 3-HP, comprising an exogenous AADH gene; an endogenous or exogenous ACC gene; an exogenous MCR gene; and an exogenous HPDH gene, and producing 3-HP through [Pyruvate Acetaldehyde&#x2192;Acetyl-CoA Malonyl-CoA Malonate semialdehyde 3-HP] biosynthesis pathway, and a method for producing 3-HP using the same.

Recombinant yeast producing 3-hydroxypropionic acid and method for producing 3-hydroxypropionic acid using the same

The present invention provides a fed-batch culture method comprising a step of fed-batch-feeding a carbon source base and a base in such a manner that the pH level can be maintained at a level suitable for the growth of microorganisms for fermentation of a carbon source. The present invention also provides a method for preparing organic acids using the fed-batch culture method. The present invention fed-batch-feeds a neutralizing agent such as ammonium bicarbonate, ammonium carbonate or alkali metal-containing weak base, and a carbon source substrate in preparing organic acids by microorganism fermentation. Thus, a pH level suitable for the survival of microorganisms for carbon source fermentation can be maintained, and the speed of injecting the carbon source base which is the source material can be appropriately adjusted. The present invention may improve productivity, yield rate and concentration of organic acids and may automatically inject a base and a carbon source substrate according to a variation in the pH level, thus improving reliability and convenience of a fermentation process operation.

Method for preparing organic acid by fed-batch-feeding carbon source substrate and base

Disclosed is a method of preparing alkyl butyrate, which comprises: (a) producing a fermented liquid containing butyrate salt through a fermentation process using butyric acid-producing strains; (b) obtaining an extracted liquid containing butyric acid from a continuous extracting apparatus using an extraction solvent, after converting the butyrate salt into butyric acid by adding an inorganic acid except for carbonic acid or carbon dioxide into the fermented liquid, wherein the continuous extracting apparatus includes a plurality of extraction plates which are installed inside an extraction column and perform a reciprocating motion vertically, and the broth and the extraction solvent are supplied to upper and lower portions of the column, respectively; and (c) reacting the extracted liquid and alcohol having a carbon number of 4 or less or a mixture thereof in an esterification reactor to convert a resultant product into alkyl butyrate. According to the present invention, butyric acid existing in microbic culture fluid can be efficiently extracted, and furthermore, competitively priced alkyl butyrate can be prepared without an additional process of separating the extracted solvent while minimizing energy consumption.

Method of preparing alkyl butyrate from fermented liquid using microorganisms

Growing community of inventors

Sejong-si, South Korea

Woo Chan Park