Massachusetts Institute of Technology

Hamamatsu-Photonics K.k.

Wonshik Choi

Michael S Feld

Christopher M Fang-Yen

Toyohiko Yamauchi

Ramachandra Rac Dasari

Zahid Yaqoob

Ramachandra Rao Dasari

The present invention relates to a full-field reflection phase microscope. In a preferred embodiment, the invention can combine low-coherence interferometry and off-axis digital holographic microscopy (DHM). The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement. A preferred embodiment of the system uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source. With improved path-length sensitivity, the present invention is suitable for full-field measurement of membrane dynamics in live cells with sub-nanometer-scale sensitivity.

Single shot full-field reflection phase microscopy

The present invention relates to systems and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle. A preferred embodiment provides tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure and the quantitative characterization of specimen-induced aberrations in high-resolution microscopy with multiple applications in tissue light scattering.

Distribution of refractive index measurement by synthetic aperture tomography

Tomographic phase microscopy

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Somerville, MA, United States of America

Wonshik Choi