USA as Represented by Secretary of the Navy

Wonmo Kang

Marc P Raphael

Christopher Rudolf

A system and method for studying cell injury mechanisms by applying biologically relevant mechanical impact to in vitro cell culture are disclosed. This approach is for maintaining consistent in vitro conditions during experiments, accommodating multiple cell populations, and monitoring each in real-time while achieving amplitude and time scale of input acceleration that mimic blunt injury cases. These multiplexed, environmental control capabilities enable characterizing the relationships between mechanical impact and cell injury in multivariate biological systems.

Vitro characterization of cell injury due to mechanical blunt

A system for mechanical testing a specimen includes a 3D printed mechanical testing fixture; a linear actuator having an axis of movement; a controller configured to control the linear actuator; two cameras; a data-acquisition system configured to acquire data from the linear actuator, the controller, and the two cameras; and the specimen. The specimen is marked in two locations with tracking markers to provide indication to the data acquisition system via at least one camera of movement and change in length of the specimen. The fixture includes force-sensing beams extending perpendicular to the axis of force.

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Tempe, AZ, United States of America

Wonmo Kang