Massachusetts Institute of Technology

National University of Singapore

Wong Cheng Lee

Krystyn J Van Vliet

Jongyoon Han

Chwee Teck Lim

Han Wei Hou

Ali Asgar Bhagat

Zhiyong Poon

The invention provides, inter alia, populations of large mesenchymal stem cells (MSC)(as well as conditioned medium from these cells) with enhanced regenerative potential, as well as methods of culturing and using these populations, such as therapeutic methods of mediating tissue repair or enhancing homing and engraftment of hematopoietic stem cells. These large MSC populations can, in certain embodiments, be produced by biophysically sorting an MSC-containing population.

Biophysically sorted osteoprogenitors from culture expanded bone marrow derived mesenchymal stromal cells (MSCs)

A method of detecting one or more diseased blood cells in a blood sample includes introducing a blood sample into at least one inlet of a microfluidic device comprising one or more linear channels wherein each channel has a length and a cross-section of a height and a width defining an aspect ratio adapted to isolate diseased blood cells along at least one portion of the cross-section of the channel based on reduced deformability of diseased blood cells as compared to non-diseased blood cells, wherein diseased blood cells flow along a first portion of the channel to a first outlet and non-diseased blood cells flow along a second portion of the channel to a second outlet. The one or more channels can be adapted to isolate cells along portions of the cross-section of the channel based on cell size. In some embodiments, the one or more channels can be spiral channels.

Microfluidics sorter for cell detection and isolation

Growing community of inventors

Singapore, Singapore

Wong Cheng Lee