Yonsei University

Won Sup Lee

No Cheol Park

Geon Lim

Guk Jong Choi

Hyungbae Moon

Sungbin Jeon

Hyung Bae Moon

Do Hyung Kim

Disclosed is a fluorescence microscope for imaging a specimen containing a fluorescent substance, the fluorescence microscope including an excitation light source configured to emit an excitation light that excites a fluorescent substance to emit fluorescence; a de-excitation light source configured to emit a de-excitation light that de-excites the fluorescent substance excited by the excitation light emitted from the excitation light source; an optical body configured to overlap a light emitted from the excitation light source and a light emitted from the de-excitation light source, and to discharge the overlapped light toward the specimen; and a solid immersion lens to which the light discharged from the optical body is incident, and configured to refract the light discharged from the optical body toward the specimen. A total internal reflection of the light incident to the solid immersion lens occurs on a bottom of the solid immersion lens.

Total internal reflection fluorescence microscope (TIRFM)

The present invention relates to a plasmonic optical waveguide using plasmonic coupling between a nano-aperture and a nano-particle. The plasmonic optical waveguide includes the nano-aperture formed with an opening of a nano-scale through which light enters; and a single metal nano-particle arranged at the focal point of the nano-aperture to generate plasmon coupling in association with the light output from the nano-aperture. The plasmonic optical waveguide has an effect of forming a small and strong high-intensity high-density light spot of a sub-wavelength size, in which an amplification rate is increased at the output surface of the nano-particle more than a few hundred times compared with the incident light, since the light is transmitted by plasmon coupling generated between the nano-aperture and the nano-particle.

Plasmonic optical waveguide using plasmonic coupling between nano-aperture and nano-particle

Growing community of inventors

Seoul, South Korea

Won Sup Lee