Boehringer Mannheim Gmbh

Wolfgang Werner

Walter Rittersdorf

Werner Guthlein

Dieter Berger

Peter Vogel

Hans Wielinger

Gunter Frey

Manfred Kuhr

Hans Lange

Peter Rieckmann

Hugo Tiedemann

Wolfgang-Reinhold Knappe

Anselm Rothe

Hans-Peter Braun

Franz Braun

Heinz Kurt Macho

Laszlo Koever

Gerd Zimmermann

Rolf Lerch

Wolfgang Vomel

Edda Geisler

Bernward Sojka

Manfred Bleisteiner

Hans-Georg Rey

Adolf K Selle

Hanz-Georg Rey

Hans-Rudolf Lange

Hugo Tiedmann

Heinz-Freidrich Trasch

Disclosed is an element for collecting, transporting and storing sample material to be analyzed containing absorptive matrix material. The element contains a first and a second layer of absorptive matrix material which are arranged side by side, next to, and touching one another on an inert support material in a contact enabling liquid transfer in such a manner that liquid can pass from the first into the second layer when the first layer is filled with liquid. The first layer can be completely separated from the second layer after applying and drying the sample material. Also disclosed is a system containing such an element and a closable envelope in which the element can be transported. In addition, the invention concerns a method for analyzing a liquid sample material in which the liquid sample material is applied to an element according to the invention, the first layer is separated from the second layer of absorptive matrix material for analysis and is subsequently eluted.

Element and system for collecting, transporting and storing sample

The present invention provides a process for the detection of redox reactions by introducing a redox reagent system into a test system, wherein a soluble iodate is additionally added to the test system in an amount which is in excess of the highest amount of disturbing reducing agents present in the test system. The present invention also provides a diagnostic agent for the detection of redox reactions containing a redox reagent system, wherein the test system used additionally contains an iodate which is soluble therein in an amount which is in excess of the highest amount of disturbing reducing agents present in the test system.

Method for the determination of redox reactions using iodate to

A device for separating plasma or serum from whole blood and analyzing the same, wherein the device includes a layer of glass fibers with an average diameter of from 0.2 to 5 microns and having a density of from 0.1 to 0.5 g./cm.sup.3 and an adsorption volume at least twice that of the plasma or serum to be separated and, preferably, more than 3.3 times the volume.

Device for separating plasma or serum from whole blood and analyzing the

Esters and agents including the esters, useful as substrates for esterolytic and/or proteolytic enzymes, said esters being compounds of the general formula: ##STR1## in which R'.sub.1, R'.sub.2, R'.sub.3 and R'.sub.4, which may be the same or different, are hydrogen atoms or lower alkyl, lower alkoxy, alkylamino or dialkylamino radicals or in which two adjacent substituents can also represent a fused benzene ring, A is a residue of an amino acid or of a peptide and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom and the method of preparation and use.

Agent for the determination of esterolytic and/or proteolytic enzymes

The present invention provides a process for the production of reagent films in which an aqueous synthetic resin dispersion which contains the usual adjuvants and reagents is coated on to a solid substrate or on to a thin fabric in a thin layer and dried, wherein to the crude film mass used for the coating there are added 0.5 to 5% by weight of a scleroprotein hydrolysate with a molecular weight of from 5000 to 50,000 and 0.5 to 10% by weight of a higher alcohol containing 5 to 10 carbon atoms.

Process for the production of reagent films and reagent films produced

The present invention provides a method of improving reagent elution from a reagent strip comprising a handle on one end which is affixed an absorbent carrier impregnated with a reagent. The absorbent carrier is pressed against the handle by a thin mesh which is stuck or sealed on to the handle on opposite sides of the carrier. Another mesh is between the carrier and the handle. When the carrier is immersed in a liquid, the meshes improve the elution of the reagent into the liquid.

Method of eluting reagent from reagent strips for chemical analyses and

Esters and agents including the esters, useful as substrates for esterolytic and/or proteolytic enzymes, said esters being compounds of the general formula: ##STR1## in which R'.sub.1, R'.sub.2, R'.sub.3 and R'.sub.4, which may be the same or different, are hydrogen atoms or lower alkyl, lower alkoxy, alkylamino or dialkylamino radicals or in which two adjacent substituents can also represent a fused benzene ring, A is a residue of an amino acid or of a peptide and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom; and the method of preparation and use.

A composition and process for permitting the separation of plasma or serum from whole blood. The composition includes glass fibers having an average diameter of from 0.2.mu. to 5.mu. and a density of 0.1 g/cm.sup.2 to 0.5 g/cm.sup.2. The process includes the steps of slowly trickling whole blood onto one side of a layer composed of the composition of glass fibers, whereby plasma or serum separated from the blood becomes available at another side of the layer. The total volume of the plasma or serum separated from the blood is limited to at most 50% of the absorption volume of the glass fiber layer.

Process and composition for separating plasma or serum from whole blood

This invention relates to new amino acid and peptide esters of leuko-indoaniline compounds and to a process for their preparation. In additional aspect, the invention relates to compositions containing such compounds for the detection of proteolytic enzymes and to methods for detecting such enzymes.

Amino acid and peptide esters of leuko-indoaniline compounds and

The detection and determination of (a) hydrogen peroxide or of a substance which reacts with the formation of hydrogen peroxide or (b) of peroxidase or a peroxidatively active substance, is carried out by contacting an unknown sample with a chromogen and, in case (a), with a peroxidatively active substance or in case (b) with hydrogen peroxide or a substance which reacts with the formation of hydrogen peroxide, and evaluating the coloration produced by the reaction, wherein, as the chromogen, there is used at least one 3,3',5,5'-tetraalkylbenzidine compound of the formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are straight-chained or branched alkyl radicals containing up to 6 and preferably up to 4 carbon atoms.

Oxidation indicators comprising 3,3',5,5'-tetraalkylbenzidine compounds

Diagnostic agent for the detection of leukocytes in body fluids, comprising an absorbent carrier which is impregnated with a chromogen and a buffer substance, wherein the chromogen is a sulfonphthalein ester of the formula ##STR1## wherein R.sub.1 is a carboxylic acid residue optionally substituted by halogen or lower alkoxy; or is an amino acid or peptide residue having a nitrogen protective group;

Diagnostic agent for the detection of leukocytes and chromogens useful

Diagnostic agent for the detection of component materials, in liquids and method of production. The diagnostic agent includes a water resistant film composed of a film-former and contains a film opener in the form of fine, insoluble inorganic or organic particles.

Diagnostic agent for the detection of component materials in liquid and

This invention relates to diagnositc agents and methods for the detection of leukocytes in body fluids. In additional aspect, the invention provides a process for the production of such diagnostic agents.

Diagnostic agents for the detection of leukocytes in body fluids

The invention provides a diagnostic agent for the detection of occult blood in feces comprising hydrogen peroxide and, as a chromogen, guaiaconic acid A, optionally with a stabilizer. The invention provides, as a novel substance, guaiaconic acid A having a specific extinction E.sub.1 cm.sup.1% at 600 nm of at least 200, determined by the reaction with peroxidase and hydrogen peroxide, having infra-red bands at

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Mannheim, Germany

Wolfgang Werner