Roche Diagnostics Gmbh

Wolfgang Treiber

Ralph Weisheit

The invention concerns a method for the determination of alkaline phosphatase in a sample by optical measurement in which interference by free haemoglobin or blood substitutes is eliminated by means of certain wavelength combinations, a method for eliminating interference caused by free haemoglobin or blood substitutes in a determination of alkaline phosphatase and the use of certain wavelength combinations to eliminate interference by free haemoglobin or blood substitutes.

Method for determining alkaline phosphatase and eliminating haemoglobin disturbances

The invention concerns a method for the determination of alkaline phosphatase in a sample by optical measurement which is characterized in that a main measurement wavelength of 450&plusmn;10 nm in combination with the rate blank procedure is used to eliminate haemoglobin interference, a method for eliminating interference by free haemoglobin or blood substitutes and the use of the combination of a main measurement wavelength with the rate blank procedure to eliminate interference by free haemoglobin or blood substitutes in the determination of alkaline phosphatase.

Method for determining alkaline phosphatase

Method and reagent for the determination of iron in a biological sample in which the bound iron is released, the released iron is reduced to Fe,, a color reagent solution is added and the color complex that is formed is measured photometrically, characterized in that a water-soluble EDTA-complexing compound in particular an indium and/or scandium salt is added to the sample.

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Weilheim, Germany

Wolfgang Treiber