Roche Diagnostics Gmbh

Wolfgang Reinhold Knappe

Rudolf Pachl

Otto Gaa

The invention concerns an analytical element for determining the amount of an analyte in a liquid containing a sample application zone ( ) and a detection zone ( ) which are in liquid-transferring contact, the latter containing at least one enzyme and an indicator substance, the enzyme catalysing a reaction in which the analyte or a substance derived from the analyte participates and the indicator substance forming a signal when the analyte is present which signal correlates with the amount of analyte, and containing a liquid-permeable interference-reducing layer ( ) in direct contact with the detection zone ( ) arranged such that liquid does not reach the detection zone until it has passed through the interference-reducing layer, the interference-reducing layer containing at least one enzyme which participates in a reaction of the analyte to be determined or of a substance derived from the analyte, wherein the analyte or the substance derived from the analyte is converted enzymatically in the interference-reducing layer into end products which cannot contribute to the signal formation by the indicator substance, and a method for the determination of an analyte in a liquid by means of such an analytical element.

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Ludwigshafen, Germany

Wolfgang Reinhold Knappe