Hoechst Schering Agrevo Gmbh

Schering Aktiengesellschaft

Basf Aktiengesellschaft

Wolfgang R Streber

Werner Boidol

Hans-Dieter Pohlenz

Meinhart H Zenk

Kenneth N Timmis

Wolf-Bernd Frommer

Frank Schmidt

Jorg Riesmeier

Olaf Ninnemann

Bernhard Grimm

Frank H&auml;nel

Jutta Papenbrock

Susanna Gr&auml;fe

Marion Kwart

The present invention relates to a nucleic acid molecule which encodes a protein with the function of a plant Mg chelatase subunit CHLD or an active fragment thereof; a protein which has the function of a plant Mg chelatase subunit CHLD or an active fragment thereof, preferably a recombinant protein; a method of determining the interaction of plant Mg chelatase subunits, in which a host cell is transformed with a DNA sequence as claimed in one or more of claims 1 to 3 and at least with one DNA sequence encoding a further subunit of Mg chelatase in such a manner that the interaction of the Mg chelatase gene products leads to a directly or indirectly, qualitatively or quantitatively measurable signal, preferably by activating a marker gene, and transgenic plants, transgenic plant cells, transgenic plant organs, transgenic plant seeds, transgenic propagation material comprising an abovementioned nucleic acid molecule.

DNA sequences encoding for subunit CHLD of plant magnesium chelatases and determining the activity of plant magnesium chelatases

The disclosure relates to the production and the use, by genetic engineering, of plasmids and bacterial strains containing, on a short, precisely characterizable DNA segment, the gene tfda or a gene almost identical to tfdA. The novel plasmids and microorganisms are suitable for the production of 2,4-D (2,4-dichlorophenoxyacetic acid)-monooxygenase, and as starting materials for the transfer, by genetic engineering of the 2,4-D-degrading property of this enzyme to various organisms.

Microorganisms and plasmids for 2,4-dichlorophenoxyacetic acid (2,4-D)

The disclosure relates to the production and the use, by genetic engineering, of plasmids and bacterial strains containing, on a short, precisely characterizable DNA segment, the gene tfdA or a gene almost identical to tfdA. The novel plasmids and microorganisms are suitable for the production of 2,4-D (2,4-dichlorophenoxyacetic acid)-monooxygenase, and as starting materials for the transfer, by genetic engineering of the 2,4-D-degrading property of this enzyme to various organisms.

Microorganisms and plasmids for 2,4-dichlorophenoxyacetic acid

Transformed plants resistant to phenmedipham, which are resistant by having been transformed with a purified and isolated carbamate hydrolase gene. The carbamate hydrolase enzyme produced thereby is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. The invention includes the transformed plants, progeny thereof, transformed plant cells, and method of producing the transformed plants and transformed plant cells, which are resistant to phenmedipham. The invention further includes plasmids comprising the carbamate hydrolase gene capable of inactivating phenmedipham.

Process for the isolation and the characterization of a gene enzyme

A process for the isolation and characterization of a gene enzyme system for the inactivation of the herbicide phenmedipham, wherein the enzyme is a carbamate hydrolase of Arthrobacter oxidans, which is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. This process includes the isolation of the carbamate hydrolase, the identification of the amino acid sequence of two BrCN cleavage peptides of the carbamate hydrolase, the synthesis of oligonucleotides for specific determination of the carbamate hydrolase sequence by hybridization and identification of the coding region, cloning and specifying the nucleotide sequence of the carbamate hydrolase gene from Arthrobacter oxidans.

Process for the isolation and characterization of a gene enzyme system

Methods for identifying substances with a potential herbicidal or

Process for the isolation and characterisation of a gene enzyme system

A process for the isolation and characterization of a gene enzyme system for the inactivation of the herbicide phenmedipham, wherein the enzyme is a carbamate hydrolase of Arthrobacter oxidans, which is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. This process includes the isolation of the carbamate hydrolase, the identification of the amino acid sequence of two BrCN cleavage peptides of the carbamate hydrolase, the synthesis of oligonucleotides for specific determination of the carbamate hydrolase sequence by hybridization and identification of the coding region, cloning and specifying the nucleotide sequence of the carbamate hydrolase gene from Arthrobacter oxidans. Plants are transformed with recombinant genes coding for the carbamate hydrolase and transgenic plants which are tolerant to the herbicide are produced.

Herbicide-tolerant plants expressing carbamate hydrolase

Growing community of inventors

Berlin, Germany

Wolfgang R Streber