Hoechst Aktiengesellschaft

Bayer Aktiengesellschaft

Boehringer Mannheim Gmbh

Wolfgang Piepersberg

Jurgen Distler

Anneliese Crueger

Ansgar Stratmann

Petra Sichel

Susanne Grabley

Hans-Georg Dellweg

Lothar Elling

Maria Regina Kula

Werner Schroder

Jurgen Georg Lenz

Brigitte Hoersch

Astrid Zervosen

Ruediger Marquardt

Andreas Seiffert-Stoeriko

Angela Weiss

J Urgen Distler

Barbara Brau

Beate Schaper

Klaus Goeke

Kambiz M Taleghani

Hermann Pape

Michael Stockmann

Michael Hemker

Juergen Distler

Barbara Br Au

Stefan Verseck

Carola Rossler

Andreas Stein

The invention relates to acarviosyl transferase from actinomycetes, mainly from Actinoplanes sp. SE 50/110 and its mutants, to a process for isolating, purifying and characterizing the enzyme, to the isolation and characterization of the acbD gene encoding the acarviosyl transferase, to the expression of the acarviosyl transferase in a heterologous host organism, to the use of the acarviosyl transferase for converting acarbose minor constituents into acarbose or for preparing acarbose homologues, to the use of the acarviosyl transferase in acarbose purification, and also to the preparation of production mutants in which formation of minor constituents is reduced by means of inactivation of the acarviosyl transferase gene.

Processes for preparing acarviosyl transferase and for using it in the

The present invention relates to processes for the enzymatic synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses starting from a nucleoside monophosphate (NMP). These processes comprise simultaneous incubation of the following substances in a buffer solution:

Process for the synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses

The invention relates to acarbose biosynthesis genes from actinomycetes, predominantly from Actinoplanes sp. SE 50/110 and its mutants, to a process for the isolation of acarbose biosynthesis genes from actinomycetes using a gene probe which has been derived from highly conserved protein regions of known dTDP-glucose dehydratase enzymes for finding the genes acbA (coding for dTDP-glucose synthase), acbB (coding for dTDP-glucose dehydratase) and acbC (coding for a cyclase, in part identical to AroB, bacterial 3-dehydroquinate synthases) or one or more acarbose biosynthesis genes from Actinoplanes sp., and to the use of the acarbose biosynthesis genes.

Acarbose biosynthesis genes from actinoplanes sp., process for the

Secondary-metabolite biosynthesis genes from actinomycetes, method of isolating them, and their use. The invention concerns secondary-metabolite biosynthesis genes from actinomycetes, a method of isolating secondary-metabolite and, in particular, 6-deoxy-sugar bio-synthesis genes, from actinomycetes using the gene probes strD, strE, strL and strM gene probes from Streptomyces griseus DSM40236, and structurally related genes, as gene probes for detecting the genes snot (coding for amphotheronolide B-dTDP-D-mycosaminyl transferase), snoD (coding for dTDP-D-glucose synthase) and snoM (coding for dTDP-4-keto-6-deoxy-D-glucose isomerase), or one or more secondary-metabolite biosynthesis genes from actinomycetes. The invention also concerns the use of secondary-metabolite biosynthesis genes thus isolated.

Secondary-metabolite biosynthesis genes from actinomycetes, method of

Process for the production of recombinant proteins in streptomycetes

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Wuppertal, Germany

Wolfgang Piepersberg