Baxalta Gmbh

Baxter Healthcare Sa

Baxter International Inc.

Immuno Aktiengesellschaft

Baxter Innovations Gmbh

Baxter Aktiengesellschaft

Takeda Pharmaceutical Company Limited

Chembiomed, Ltd.

Nanotherapeutics, Inc.

Bexter Healthcare S.a.

Baxalta Innovations Gmbh

'Immuno' Aktiengesellschaft Fur Chemisch-Medizinische

Immuno Aktiengesellschaft Fur Chemisch-Medizinische Produkte

Wolfgang Mundt

Manfred Reiter

Friedrich Dorner

Leopold Grillberger

Artur Mitterer

Noel Barrett

Otfried Kistner

Johann J Eibl

Christa Tauer

Peter Turecek

Hans-Peter Schwarz

Bernhard Fischer

Falko-Guenter Falkner

Uwe Schlokat

Renate Den-Bouwmeester

Meinhard Hasslacher

Barbara Kraus

Thomas R Kreil

Horst Schafhauser

Simone Von Fircks

Simone Feigl

Richard H Smith

Christa Mayer

Heidi Meyer

Uwe B Sleytr

Axel Bruehmann

Paul Messner

Frank M Unger

Wilfried Woehrer

Leopold Gillberger

James L Mcdonel

Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.

Method of producing recombinant high molecular weight vWF in cell culture

The invention relates to a method for removing a viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. The method comprises subjecting said preparation to filtration for at least about 24 hours through a virus filter having an effective pore size of maximum about 75 nm. Further, the invention relates to the use of a virus filter in filtration of at least about 24 hours, wherein the virus filter has an effective pore size of maximum about 75 nm for the removal of viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. In some embodiments the filtration according to the invention operates at a volumetric capacity of at least about 2000 L/m. Further, the invention relates to the use of a preparation, being a cell culture medium or at least a component of a cell culture medium obtainable according to method of the invention for cell culture; pharmaceutical, diagnostic and/or cosmetic preparations as well as in food preparations.

Virus filtration of cell culture media

The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.

Oligopeptide-free cell culture media

The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

Animal protein-free media for cultivation of cells

Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.

Recombinant cell clones having increased stability and methods of making and using the same

A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.

Medium for the protein-free and serum-free cultivation of cells

The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.

Formulation of sugar solutions for continuous ultracentrifugation for virus purification

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Vienna, Austria

Wolfgang Mundt