Roche Diagnostics Gmbh

Wolfgang Ludwig

Anne Stern

Christoph Kessler

Karl-Heinz Schleifer

Ruediger Rueger

For the genus-specific or/and species-specific detection of bacteria in a sample liquid, bacterial RNA is hybridized with a primer which is complementary to a genus-specific or species-specific region of the RNA of particular bacteria or to a highly conserved region of the RNA of bacteria in general, but which is not complementary at its 3&prime; end to the RNA of bacteria of another genus or species, the primer is elongated in the presence of a suitable polymerase and the four deoxyribonucleotides, if desired, with a concurrent or subsequent labelling of the elongation product and an elongation product formed is hybridized with a genus-specific or species-specific oligonucleotide after denaturation and the hybridization is detected by means of the oligonucleotide label.

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Sachsenkam, Germany

Wolfgang Ludwig