Life Technologies Corporation

Life Technologies As

Raindance Technologies, Inc.

Ion Torrent Systems Incorporated

Bio-rad Laboratories, Inc.

Other

Wolfgang Hinz

Jonathan Marc Rothberg

James M Bustillo

Kim L Johnson

John Harris Leamon

Steven Menchen

Geir Fonnum

Shifeng Li

Astrid Evenroed Molteberg

David Light

Carl Williams Fuller

John F Davidson

Alexander J Mastroianni

Nini Kjus

Grete Irene Modahl

Luisa Andruzzi

Jeremy Gray

Marc Glazer

James A Ball

Darren Roy Link

Jonathan M Schultz

Michael Weiner

David Marran

David Cox

Christina E Inman

Scott Parker

Phil Waggoner

Todd Rearick

Mark James Milgrew

Dmitriy Gremyachinskiy

Kristopher Barbee

Paul M Kenney

Yufang Wang

Ronald L Cicero

Scott C Benson

Earl A Hubbell

Peter B Vander Horn

Prasanna Krishnan Thwar

Daniel Beacham

Keith Glen Fife

Barnett B Rosenblum

Ronald J Graham

Kai Qin Lao

Bin Li

Shaheer Khan

Jo Aaserud

M Talha Gokmen

John Q Lu

Gilbert Feke

Laurent Boitard

Jeffrey Branciforte

Yves Charles

Ahmadali Tabatabai

Diem Tran

Antoine Van Oijen

Michael L Minto

Kimberly Gorrell

John Matthew Mauro

Roman Rozhkov

Martin Huber

Talha M Gokmen

Diem Thuy Thi Tran

Christian Woehler

Alfred Lui

Lily Lu

Ryan Jones

John Donohue

Synne Larsen

Lene Husaboe

Elisabeth Breivold

David Ruff

Shiaw-Min Chen

Rachel Kasinskas

Jennifer O'Neil

Melville Davey

Chieh-Yuan Li

Jonathan O Schultz

Craig Stolarczyk

Pontus Lundberg

Richard Whitaker

Muhammed Talha Gokmen

Jonathan M Rothberg

Tanya Sokolsky

Bradley Hann

Philip Waggoner

Johnathan M Rothberg

Grete Modahl

Steven M Menchen

Antoine M Van Oijen

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

Scaffolded nucleic acid polymer particles and methods of making and using

Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

Methods and apparatus including array of reaction chambers over array of chemFET sensors for measuring analytes

The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

Microfluidic devices and methods of use in the formation and control of nanoreactors

Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.

Cell analysis using ChemFET sensor array-based systems

Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

Methods and apparatus for measuring analytes using large scale FET arrays

A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

Surface stabilization of biosensors

A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

Hydrophilic polymeric particles and methods for making and using same

Integrated sensor arrays for biological and chemical analysis

A method of forming a polymer matrix array includes treating a surface within a well of a well array with a surface compound including a surface reactive functional group and a radical-forming distal group; applying an aqueous solution including polymer precursors to the well of the well array; and activating the radical-forming distal group of the surface coupling compound with an initiator and atom transfer radical polymerization (ATRP) catalyst to initiate radical polymerization of the polymer precursors within the well of the well array to form the polymer matrix array.

Matrix arrays and methods for making same

Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

Growing community of inventors

Killingworth, CT, United States of America

Wolfgang Hinz