Merck Patent Gesellschaft MIT Beschrankter Haftung

Wolfgang Brummer

Wolfgang Ebeling

Norbert Hennrich

Michael Klockow

Roland Helger

Hermann Lang

Harald Metz

Martin Heinrich

Hans Dieter Orth

Dieter Banauch

Gunter Schmid

Ulrich Behrendt

The invention relates to a process for achieving high, thermally-induced expression rates, which last for a relatively long period of time, of proteins in recombinant cells, preferably in E. coli cells, by optimizing cell density, induction temperature and supplemented amino-acid-containing substrates.

Overexpression of proteins in recombinant host cells

The invention relates to a process for the microbiological preparation of aldose-1-epimerase by cultivating microorganisms in a nutrient medium and release of the enzyme from the cells, which process is characterized in that microorganisms of the genus Acinetobacter are used.

Process for the microbiological preparation of aldose-1-epimerase

Glucose is rapidly ad quantitatively determined with an analytical agent containing glucose dehydrogenase having an activity of at least 2 .mu./mg and NADH.sub.2 oxidase activity less than 0.1%, a pyridine coenzyme, a buffer and mutarotase to increase spontaneous mutarotation of alpha-glucose to beta-glucose. There may be optionally present inhibitors for reduced pyridine coenzyme oxidases and a thermal stabilizing amount of alkali metal chloride.

Enzymatic determination of glucose

Biochemically active compounds e.g., proteins and particulate biological materials such as bacteria and viruses, are immobilized on a water-swellable or water-insoluble matrix by covalent bonding to a partially crosslinked polymer containing reactive groups capable of binding the biochemically active compound thereto.

Carrier matrix for the fixation of biochemically effective substances

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Alsbach-Hahnlein, Germany

Wolfgang Brummer