Roche Sequencing Solutions, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Wojtek Steffen

Victor I Lyamichev

Frank Bergmann

Michael Schraeml

Jigar Patel

Thomas J Albert

Alfred Engel

Thomas Streidl

Tobias Oelschlaegel

Mohamed Yosry Hassan Mohamed

Thomas Albert

Romina Eisenhauer

Michael Schaeml

In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.

System and method for identification and characterization of transglutaminase species

According to one aspect, the present disclosure provides a method of identifying a substrate of a transglutaminase using a peptide array comprising a plurality of peptides. The method includes the steps of contacting the peptides in the peptide array with the transglutaminase, allowing the transglutaminase to bind to the peptides, and identifying the substrate of the transglutaminase.

Identification of transglutaminase substrates and uses therefor

In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases.

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Penzberg, Germany

Wojtek Steffen