Alfacell Corporation

Tamir Biotechnology, Inc.

National Institutes of Health, a Component of the US Dept. of Health & Human Services

Wojciech J Ardelt

Stanislaw M Mikulski

Shailendra K Saxena

Richard J Youle

Veena M Vasandani

Ester Boix

Yon-Neng Wu

Kuslima Shogen

Three RNases (ranpirnase, the second embodiment disclosed in U.S. Pat. No. 5,728,805, and recombinant Amphinase-2) are tested against identified herpesviridae infections. With some exceptions, quantitative PCR assays indicate that the RNases have anti-viral activity against many of these viruses.

Methods of treating infections originating from viruses in the herpesviridae family

Two RNases (ranpirnase and the second embodiment disclosed in U.S. Pat. No. 5,728,805) are tested against human papillomavirus infections. QRT-PCR assays indicate that the RNases have anti-viral activity against type 11 HPV.

Methods of treating human papillomavirus

The present invention provides recombinant Onc (rOnc) compositions and methods. Recombinant Onc proteins of the invention have an amino terminal methionine and comprise an Onc polypeptide. The amino terminal methionine of the protein allows for recombinant production in a bacterial host cell. Cleaving the amino terminal methionine exposes the amino terminal glutamine of the polypeptide. The Onc polypeptide has an amino terminal glutamine. Cyclization of the amino terminal glutamine of the polypeptide to a pyroglutamyl residue provides rOnc polypeptides and proteins have anti-cancer and anti-viral activity.

Mutant form of a cytotoxic ribonucleolytic protein which allows production by recombinant methods

Mutant form of cytotoxic ribonucleolytic protein which allows production by recombinant methods

A protein family includes four proteins that are bioactive against human tumor cell lines. The proteins are derived from eggs of the,frog, and are members of the superfamily of pancreatic ribonucleases.

Family of proteins belonging to the pancreatic ribonuclease a superfamily

Rana pipiens eggs are subjected to mechanical processing in the presence of a weakly acidic buffer to produce an extract. The extract is subjected to ion-exchange chromatography and size-exclusion chromatography. Two pharmaceuticals resulting from these steps have activity against certain cancer cells. The amino acid sequences and compositions of two preferred embodiments are disclosed.

Pharmaceuticals and method for making them

A pharmaceutical known by the trademark ONCONASE, as described in pending commonly owned application application number 07/436,141 filed Nov. 13, 1989, is combined with two forms of another drug known as Lovastatin. The combination of ONCONASE with Lovastatin has unexpected bioactivity in vitro against ASPC-1 human pancreatic adenocarcinoma cells, A-549 human lung carcinoma cells and HT-520 human squamous cell lung carcinoma cells.

Compositions comprising ONCONASE (tm) and lovastatin

Rana pipiens eggs are subjected to fertilization and the embryos are grown to a predetermined stage of development. The embryos and unfertilized eggs are then subjected to mechanical processing in the presence of a weakly acidic buffer to produce an extract. The extract is subjected to ion-exchange chromatography and size-exclusion chromatography. The resulting pharmaceutical has activity against certain cancer cells. The amino acid sequence and composition of the pharmaceutical are disclosed.

Frog embryo and egg-derived tumor cell anti-proliferation protein

A pharmaceutical to be sold under the ONCONASE trademark, as described in pending commonly owned application application Ser. No. 07/436,141 filed Nov. 13, 1989 is combined with other drugs sold under the trademarks TAMOXIFEN and STELAZINE. The combination of ONCONASE with TAMOXIFEN has unexpected bioactivity in vitro against ASPC-1 human pancreatic adenocarcinoma cells and the combination of ONCONASE with STELAZINE has unexpected bioactivity in vitro against A-549 human lung carcinoma cells.

Compositions comprising ONCONASE (TM) and STELAZINE (TM) or TAMOXIFEN

A pharmaceutical known by the trademark ONCONASE, as described in pending commonly owned application application Ser. No. 07/436,141 filed Nov. 13, 1989, is combined with drugs sold under the names cisplatin, Melphalan and Doxorubicin HCl. The combinations of ONCONASE with these drugs has unexpected bioactivity in vitro against OVCAR-3 human ovarian adenocarcinoma cells.

Compositions comprising ONCONASE(.TM.) and cisplatin, melphalan, or

Rana pipiens eggs are subjected to fertilization and the embryos are grown to a predetermined stage of development. The embryos and unfertilized eggs are then subjected to mechanical processing in the presence of a weakly acidic buffer to produce an extract. The extract is subjected to ion-exchange chromatography and size-exclusion chromatography. The resulting pharmaceutical has activity against certain cancer cells. A partial amino acid sequence of the pharmaceutical is disclosed.

Growing community of inventors

New City, NY, United States of America

Wojciech J Ardelt