Roche Sequencing Solutions, Inc.

Genia Technologies, Inc.

Wiseley Wu

Mark R Ambroso

Timothy Kellogg Craig

Corissa Harris

Marshall Winston Porter

Yaozhong Zou

Matt Dipetro

Issa Delkaninia

Alexxa Noble

James Fairman

Kapil Bajaj

Giovanni Bellesia

Seong-Ho Shin

Timothy Craig

Described herein are nanopore protein conjugates that can be used in DNA sequencing reactions. The nanopore protein conjugates includes a nanopore protein monomer that is joined to a DNA binding domain. The nanopore protein monomer is available to oligomerize with other nanopore protein monomers, while the DNA binding domain is available to bind to a template DNA strand. In certain examples, the nanopore protein monomer is an alpha-hemolysin monomer or variant thereof and the DNA binding domain is an Sso7d protein or variant thereof, such as an Sso7d-like protein. Also provided are nanopore protein assemblies incorporating the nanopore protein conjugates, along with methods of using the nanopore protein assemblies in sequencing reactions.

Nanopore protein conjugates and uses thereof

Growing community of inventors

Newark, CA, United States of America

Wiseley Wu