Emd Millipore Corporation

Millipore Corporation

Entegris, Inc.

Millipore Investment Holdings, Ltd.

Merck Patent Gmbh

Mykrolis Corporation

Wilson Moya

Mikhail Kozlov

Neil P Soice

Jad Jaber

Gabriel Tkacik

Volkmar Thom

Anthony J DiLeo

Michael W Phillips

Sarah Yuan

Justin McCue

Igor Quinones-Garcia

Philip M Goddard

John Charkoudian

James Hamzik

Nripen Singh

Brian Gagnon

Senthilkumar Ramaswamy

Yu Zhang

Arezki Boudif

Sherry Ashby Leon

Jibin Hao

Onur Y Kas

Phillip Clark

Matthew T Stone

Ajish Potty

David DeCoste

William Cataldo

Romas Skudas

John Saragosa

Christopher Gillespie

George Gagne

Paul J Cook

Sven Andrecht

Benjamin Cacace

Alex Xenopoulos

Bianca Edelmann

Alison Dupont

Klaus Adrian

Bianca Edlemann

Stefano Berti Perez

Shannon Cleveland

Marina Varlamova

Daniel Lamothe

James Dee

A biocontainer having a first film, the film having an interior and exterior side; articulating elements disposed on or within the first film, the articulating elements comprising at least one a folded hinge, a sealed joint, a thinned pathway, a bowed path, an embedded polymeric or metallic cylindrical fiber or rod; and a second film, optionally comprising articulating elements, joined to the first film, to form a biocontainer having a closed volume, wherein the articulating elements permit the biocontainer to expand and collapse along the articulating elements.

Articulating biocontainers

The embodiments described herein relate to agarose ultrafiltration membrane composites and methods for making and using the same.

Agarose ultrafiltration membrane composites for size based separations

The present invention relates to improved processes and systems for purification of biological molecules, where the processes can be performed in a continuous manner.

Purification of biological molecules

The present invention relates to the purification of target molecules like recombinant and/or biotherapeutic proteins. Activated carbon can be used to remove leachables and/or extractables resulting from disposable equipment employed in the process.

Activated carbon for the removal of leachables and/or extractables

The present invention relates to a selectively soluble polymer capable of binding to one or more constituents in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and is rendered insoluble and precipitates out of solution upon a change in the process conditions. While in its solubilized state, the polymer is capable of binding to a selected entity within the stream such as impurities (DNA, RNA, host cell protein, endotoxins, etc.) in a cell broth and remains capable of binding to that entity even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered and further processed.

Purification of proteins

A method for removing microorganisms from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention. Microorganisms such as bacteria, particularly, are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having aLRV greater than about 9, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. Another method for removing microorganisms such as bacteria and, includes passing the liquid through a porous nanofiber containing filtration medium having a microorganism LRV greater than about 8, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. The filtration medium can be in the form of a fibrous electro spun polymeric nanofiber liquid filtration medium mat.

Removal of microorganisms from fluid samples using nanofiber filtration media

A method for removing retroviruses from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention is disclosed. Retroviruses are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having a retrovirus LRV greater than about 6, and the nanofiber(s) has a diameter from about 10 nm to about 100 nm. The filtration medium can be in the form of a fibrous electrospun polymeric nanofiber liquid filtration medium mat.

Method for retrovirus removal

The present invention relates to a selectively soluble polymer capable of binding to a desired molecules in an unclarified mixture containing various biological materials and the methods of using such a polymer to purify a molecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired molecule (protein, polypeptide, etc) and remains capable of binding to that molecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.

Container for sample preparation or processing, such as biomass culturing or processing, and optionally sample purification. In certain embodiments, the reactor is a bioreactor that includes a stirred cell device that simulates a tangential flow filter to reduce or eliminate clogging that can be caused by the solids generated. In certain embodiments, the solids comprise a precipitate or floc or beads, such as one that includes a polymer that binds the biomolecule(s) of interest, and impurities. In its method aspects, embodiments disclosed herein include purification and isolation of biomolecules of interest derived from cell culture fluids. The methods include carrying out sample preparation or processing in a container, culturing a biomass; generating solids by precipitating or flocculating a biomolecule of interest from the cultured broth; preventing the solids from settling in the container by agitation; and purification, such as by eluting the biomolecule of interest and filtering the same.

Stirred tank reactor and method

The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.

Stimulus responsive polymers for the purification of biomolecules

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Concord, MA, United States of America

Wilson Moya