Other

Aventis Pasteur Limited

Willie Wei Qiang Sun

Richard C Egen

Lori Ann Fortin

Animal-free meninge fermentation media and process is developed based upon use of a chemically defined medium. To improve polysaccharide production, fed-batch fermentation is examined using different feed solutions and feeding strategies. A feed solution containing glucose, amino acids, and trace metal elements produces Group A polysaccharide at approximately 3 times the level observed with batch fermentation. This process is used successfully to produce polysaccharides ofserotypes A, C, Y and W-135 and is run reproducibly at the 20L scale and can be scaled to 400L or more.

Animal component free meningococcal polysaccharide fermentation and seedbank development

Animal-free meninge fermentation media and process is developed based upon use of a chemically defined medium. To improve polysaccharide production, fed-batch fermentation is examined using different feed solutions and feeding strategies. A feed solution containing glucose, amino acids, and trace metal elements produces Group A polysaccharide at approximately 3 times the level observed with batch fermentation. This process is used successfully to produce polysaccharides ofserotypes, A, C, Y and W-135 and is run reproducibly at the 20 L scale and can be scaled to 400 L or more.

Growing community of inventors

Easton, PA, United States of America

Willie Wei Qiang Sun