Inscripta, Inc.

William Watterson

Phillip Belgrader

Jorge Bernate

Eric D Smith

Bruce Chabansky

Christian Siltanen

David Stumbo

Burak Dura

A massaging pillow assembly includes a pillow that has a plurality of pouches each extending into an interior of the pillow to be accessed by a user. A vibration unit is insertable into a respective one of the pouches such that the vibration unit is in mechanical communication with the pillow. The vibration unit generates a vibration when the vibration unit is turned on thereby communicating the vibration into the pillow to soothe the user with the vibration. A pair of pads is each of the pads is coupled to the pillow. Each of the pads is comprised of a friction producing material to inhibit the pillow from sliding. A pair of cup holders is each removably attachable to the pillow to hold a beverage container.

Massaging pillow assembly

Nucleic acid-guided nuclease editing in mammalian cells may include passaging mammalian cells, in an automated closed cell editing instrument, into smaller aggregates when the aggregates exceed 50-300 microns in size. A library of viral particles may be delivered to the mammalian cells at a multiplicity of infection such that each mammalian cell receives one or no viral particle. The library may include viral vectors with an editing cassette including a pair of gRNA coding sequence and donor DNA. Conditions may be provided to allow a viral vector of the viral vectors to integrate into the mammalian cells. Enriching for mammalian cells may be done with an integrated viral vector. A nucleic acid-guided nuclease or nuclease fusion or a coding sequence for a nucleic acid-guided nuclease or nuclease fusion may be delivered to the enriched mammalian cells and conditions may be provided to allow editing in the mammalian cells.

Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells via viral delivery

This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells. The present compositions and methods entail viral delivery of an editing cassette to live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow, preferably using a fully-automated end-to-end instrument to process the cells without human intervention to enhance cell processing uniformity and to maintain the integrity of the cell culture.

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Boulder, CO, United States of America

William Watterson