University of Illinois

Biotechnology Research and Development Corporation

William W Metcalf

Wilfred A Van Der Donk

Huimin Zhao

Ryan Woodyer

Benjamin Thomas Circello

Jiangtao Gao

Svetlana A Borisova

Tyler Johnannes

James R Doroghazi

Dipti Dinkar Nayak

Kou-San Ju

Alexander L Polidore

Junkal Zhang

David P Labeda

Here we show thatproduces at least three phosphonates, two of which were purified and structurally characterized. The first, designated pantaphos, was shown to be 2-(hydroxy(phosphono)methyl)maleate; the second, a probable biosynthetic precursor, was shown to be 2-(phosphonomethyl)maleate. Purified pantaphos is both necessary and sufficient for the hallmark lesions of onion center rot. Moreover, when tested against mustard seedlings, the phytotoxic activity of pantaphos was comparable to the widely used herbicides glyphosate and phosphinothricin. Pantaphos was also active against a variety of human cell lines.

Phosphonate products and methods

Methods of RNA-guided DNA endonuclease-mediated genome editing in Bacteria and Archaea are provided.

Genome editing in Archaea

The present invention relates to pharmaceutical compositions comprising a phosphonic acid compound, such as a natural product. Such compounds can include, such as an antibiotic or compound with other activity, derived from anstrain having a gene encoding pepM (phosphoenolpyruvate phosphomutase) or a pepM-dependent biosynthetic pathway. The present invention also relates to methods for treating or preventing or reducing the risk of a bacterial infection by administering a therapeutically effective or prophylactically effective amount of a phosphonic acid antibiotic, or a pharmaceutical composition containing such an antibiotic, to a patient or subject in need thereof. The present invention further relates to methods for isolating, purfying, and identifying such phosphonic acid compounds fromstrains.

Phosphonic acid compounds and screening method

The disclosure of the present application provides polypeptide sequences and nucleotide sequences coding for the polypeptide sequences of proteins used in the production of APPA-containing peptides. In at least one embodiment of the present disclosure, an isolated nucleic acid is disclosed which comprises a nucleotide sequence encoding a polypeptide having a sequence identity of 60 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS: 2-13, and 15-23.

Compositions and methods for the synthesis of APPA-containing peptides

Phosphite dehydrogenase mutant enzymes were generated that provide relaxed cofactor specificity and increased thermostability over the wild type enzyme. The mutant enzymes are useful for nicotinamide cofactor regeneration.

Phosphite dehydrogenase mutants for nicotinamide cofactor regeneration

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Savoy, IL, United States of America

William W Metcalf