Zymergen Inc.

William W Hwang

Jeffrey Kim

Oliver Liu

Jennifer Shock

Zachariah Serber

Alexander Neckelmann

Xiao Yang

Eyal Akiva

Stephanie Leanne Brown Hendrix

Samuel Oteng-Pabi

Tom Hayon Eyles

Claus Pelikan

Ute Galm

Devin Scannell

Sangita Ganesh

Michael Sneddon

The present disclosure provides methods and systems for identifying natural product-encoding multi-gene clusters (MGCs). In some embodiments, the present disclosure also teaches methods for producing sequenced and assembled metagenomic libraries that are amenable to MGC search bioinformatic tools and techniques.

Metagenomic library and natural product discovery platform

The present disclosure provides methods and systems for identifying natural product-encoding multi-gene clusters (MGCs). In some embodiments, the present disclosure also teaches methods for producing sequenced and assembled metagenomic libraries that are amenable to MGC search bionformatic tools and techniques.

The disclosure presents a platform for discovering novel insecticidal proteins from highly heterogeneous environmental sources. The methodology utilizes metagenomic enrichment procedures and unique genetic amplification techniques, which enables access to a broad class of unknown microbial diversity and their resultant proteome. The disclosed insecticidal protein discovery platform (IPDP) can be computationally driven and is able to integrate molecular biology, automation, and advanced machine learning protocols. The platform will enable researchers to rapidly and accurately access the vast repertoire of untapped insecticidal proteins produced by uncharacterized and complex microbial environmental samples. Also presented herein are a group of newly discovered pore-forming toxins (PFT) from a rare class of insecticidal proteins, which were discovered utilizing the insecticidal protein discovery platform.

Insecticidal protein discovery platform and insecticidal proteins discovered therefrom

Growing community of inventors

El Cerrito, CA, United States of America

William W Hwang