Southern Research Institute

William W Barrow

Thomas P Dooley

William J Suling

Sabrina Z Van Ginkel

Jay K Staas

Gary A Winchester

Debra C Quenelle

Esther L Barrow

The invention relates to the nucleic acid sequence and amino acid sequence of dihydrofolate reductase (DHFR) from mycobacteria and to expression of recombinant DHFR protein. Utilizing the recombinant protein, novel therapies and diagnostic strategies can be developed and selective antimycobacterial compositions can be designed and utilized to treat mycobacterial infections in patients. This invention includes all or portions of novel recombinant nucleic acids encoding DHFR for mycobacteria such as , to novel recombinant DHFR peptides produced by such sequences, and to vaccines, diagnostic kits, cells and therapies utilizing these peptides and nucleic acid sequences. The present invention relates to methods for using the sequences of the present invention to develop drugs specific to and other mycobacteria, to identify and sequence corresponding sequences in species other than , as well as diagnostic and treatment methods incorporating the disclosed sequences and peptides.

Mycobacterium fol A gene that encodes for the enzyme dihydrofolate reductase

A method of treating or preventing an intracellular infection in an animal comprising administering a first effective amount of a suitable drug contained in first biocompatible microspheres that have a diameter of less than or equal to about 10 microns, wherein the first microspheres release the suitable drug upon administration at a first effective rate. A second set of microspheres greater than 10 microns in diameter may also be administered to provide continuing systemic release of the drug.

Compositions and methods for treating intracellular infections

The invention relates to the nucleic acid sequence and amino acid sequence of dihydrofolate reductase (DHFR) from mycobacteria and to expression of recombinant DHFR protein. Utilizing the recombinant protein, novel therapies and diagnostic strategies can be developed and selective antimycobacterial compositions can be designed and utilized to treat mycobacterial infections in patients. This invention includes all or portions of novel recombinant nucleic acids encoding DHFR for mycobacteria such as,, to novel recombinant DHFR peptides produced by such sequences, and to vaccines, diagnostic kits, cells and therapies utilizing these peptides and nucleic acid sequences. The present invention relates to methods for using the sequences of the present invention to develop drugs specific to,and other mycobacteria, to identify and sequence corresponding sequences in species other than,, as well as diagnostic and treatment methods incorporating the disclosed sequences and peptides.

Mycobacterium fol a gene that encodes for the enzyme dihydrofolate reductase

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Hoover, AL, United States of America

William W Barrow