University of Medicine and Dentistry of New Jersey

Alliedsignal Inc.

William T McAllister

Jon I Williams

Ina Goldberg

John F Klement

Anthony J Salerno

A cloned DNA sequence encoding T3 RNA polymerase is provided. This DNA can be inserted into an expression vector for recombinant expression of the peptide. The peptide can be used with dual promotor vectors containing T3 specific promoters.

Gene coding for a protein having T3 polymerase activity

This invention relates to processes for the microbial production of peptide oligomers, to polypeptide products resulting from application of any of these processes, and to microbes for use in such production. Another aspect of this invention relates to processes for genetically engineering such microbes and to plasmid vectors for use in such engineering.

Method of producing a gene cassette coding for polypeptides with

A dual phage RNA polymerase promoter having a polylinker with at opposite ends, a T3 phage RNA polymerase promoter and a phage RNA polymerase promoter, like T7; the promoters are linked to the polylinker in opposite orientation. A recombinant DNA vector containing a T3 phage RNA polymerase promoter and a different phage RNA polymerase promoter; the promoters are linked to a polylinker sequence in opposite orientation. A kit including a T3 promoter and containing appropriate components for synthesizing RNA transcripts that are complementary to either strands of a cloned DNA sequence, and for other applications. Gene coding for T3 RNA polymerase, a vector containing the gene and transformed microorganisms.

Plasmid for the overproduction of bacteriophage T3 RNA polymerase,

A dual promoter cassette which has at one end a promoter for T3 RNA polymerase which contains a downstream sequence identical to a naturally occurring T3 promoter sequence and on the other end, a promoter for a phage DNA polymerase other than the T3 RNA polymerase. The recombinant DNA plasmid which includes the promoter. The plasmid is capable of highly efficient transcription of RNA with low concentrations of ribonucleoside triphosphates.

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Metuchen, NJ, United States of America

William T McAllister