Zymergen Inc.

William Serber

Erik Jedediah Dean

Sean Poust

Sara Da Luz Areosa Cleto

Philip Weyman

Vytas SunSpiral

Matthew Jonathan Myers

The present disclosure provides a microbe engineering platform that permits optimization of microbe fitness levels to optimize a microbe's suitability for industrial fermentation. The disclosed platform identifies an association between microbe properties and microbe fitness levels. The association between microbe properties and microbe fitness levels may be used to identify candidate microbes with desired fitness levels. The identified candidate microbes may be used to further optimize the industrial fermentation process.

Microbial engineering methods and systems for optimizing microbe fitness

Aspects of this disclosure relate to liquid-based workflows for strain engineering. In strain engineering, cells undergo a modification process to acquire a desired change or changes in the cells. Some of the cells accept the change or changes and some do not. Provided herein are liquid-based methods for selecting the population of cells that have accepted the change or changes. Also provided herein are liquid-based methods for removing markers from transformed cells and liquid-based methods for isolating clonally pure populations of cells.

Liquid based selection and cell isolation

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Berkeley, CA, United States of America

William Serber